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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

Plasmid

#111818

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Mef2c (Mef2c Mouse)
Tdtomato
Gata4 (Gata4 Mouse)
Tbx5

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HIP1R-GFP-FKBP

Plasmid

#100752

Purpose

Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

HIP1R (HIP1R Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 Q331K

Plasmid

#27454

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pTEC17

Plasmid

#30176

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Teal

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

Plasmid

#111818

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Mef2c (Mef2c Mouse)
Tdtomato
Gata4 (Gata4 Mouse)
Tbx5

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HIP1R-GFP-FKBP

Plasmid

#100752

Purpose

Expression of HIP1R with a GFP-FKBP tag in mammalian cells.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

HIP1R (HIP1R Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol2p2A--3mnx1:mTagBFP-NS-MCS

Plasmid

#78250

Purpose

Can be used to subclone in gene of interest into blue motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-mTagBFP-NS-MCS

Use

Zebrafish motor neuron expression

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 Q331K

Plasmid

#27454

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

Plasmid

#169128

Purpose

AAV plasmid containing a mKate2 and a truncated PDE4D3

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Has Service

AAV1

Insert

mKate2-PDE4DCatL (PDE4D Human)

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

truncated, Catalatic domain only

Promoter

EF1a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTEC17

Plasmid

#30176

Depositor

Lalita Ramakrishnan

Article

Takaki et al Nat Protoc. 2013 Jun;8(6):1114-24. doi:

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Teal

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

Plasmid

#169128

Purpose

AAV plasmid containing a mKate2 and a truncated PDE4D3

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Has Service

AAV1

Insert

mKate2-PDE4DCatL (PDE4D Human)

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

truncated, Catalatic domain only

Promoter

EF1a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC337 - pAAV EF1a DIO mCherry

Plasmid

#47636

Purpose

An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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IL2 pAcGP67a

Plasmid

#41806

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

IL2 (IL2 Human)

Tags

6x His

Expression

Insect

Promoter

Polyhedron

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pLMB509

Plasmid

#40084

Depositor

Philip Poole

Article

Tett et al Appl Environ Microbiol. 2012 Oct;78(19):

Inserts

gfpmut 3.1
tauR
tau promoter

Expression

Bacterial

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pOTTC337 - pAAV EF1a DIO mCherry

Plasmid

#47636

Purpose

An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

mCherry

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge