We narrowed to 6,438 results for: WUS;

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pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

Plasmid

#169186

Purpose

To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86iso1

Plasmid

#223601

Purpose

Lentiviral expression of human CD86 isoform 1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 isotype1 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-MTHFD2

Plasmid

#31213

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

MTHFD2 (MTHFD2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR1A-TAZ S89A

Plasmid

#179911

Purpose

Entry vector of TAZ S89A for gateway coloning.

Depositor

Xin Chen

Article

Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

Insert

WW Domain Containing Transcription Regulator 1 (WWTR1 Human)

Use

Entry vector

Tags

3X Flag-tag

Mutation

Serine 89 mutated to Alanine

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD86

Plasmid

#223598

Purpose

Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

Plasmid

#223213

Purpose

Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

TGFb1 active - membrane (TGFB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-FLAG-YFP-KIBRA

Plasmid

#235683

Purpose

Express YFP tagged KIBRA gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

KIBRA (WWC1 Human)

Use

Lentiviral

Tags

FLAG-YFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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FKBP:FRB-FBAR

Plasmid

#223620

Purpose

Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

FBP17 (FNBP1 Human)

Tags

FKBP, TagBFP and mGFP, FRB

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PDL1

Plasmid

#223602

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

Plasmid

#193968

Purpose

Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

hPol eta (POLH Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

Plasmid

#187587

Purpose

Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SMN2 (SMN2 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-p53 (1-90) (72R)

Plasmid

#62081

Purpose

Expression of human p53 (TAD residues 1-90) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains TAD/PP residues 1-90, 72R

Promoter

tac

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only
pHR-CD80-mTFP1

Plasmid

#223605

Purpose

Lentiviral expression of human CD80 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-K-GECO1

Plasmid

#105864

Purpose

Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Tags

6-His Tag

Expression

Bacterial

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-Bi-DREADD

Plasmid

#159457

Purpose

AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

Bi-DREADD

Expression

Mammalian

Mutation

fusion protein of hM3Dq-mCherry and HA-KORD seper…

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-human HoxB4-IRES-YFP

Plasmid

#91889

Purpose

Retroviral expression of human HoxB4

Depositor

Martine Roussel, Brian Sorrentino

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

HoxB4 (HOXB4 Human)

Use

Retroviral

Tags

IRES-EYFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGImC_V5-CSNK1A1

Plasmid

#92014

Purpose

Expresses V5-CSNK1A1, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-mGFP-Eps15DN

Plasmid

#223621

Purpose

Transient expression of human Eps15 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

Eps15 (EPS15 Human)

Tags

mGFP

Expression

Mammalian

Mutation

Eps15 _ 95-295aa

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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hyA3A-BE4max

Plasmid

#157943

Purpose

Mammalian expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyA3A-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A-NG

Plasmid

#220895

Purpose

Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A-NG

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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