We narrowed to 8,727 results for: pcDNA3

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K27 histone methylation reporter

Plasmid

#22865

Depositor

Alice Ting

Article

Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

Insert

K27 HMR

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-rat PKA-RIIbeta-mEGFP

Plasmid

#45523

Depositor

Haining Zhong

Article

Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

Insert

PKA regulatory subunit RII beta tagged by mEGFP

Expression

Mammalian

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag HBx (G124L, I127A)

Plasmid

#42598

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X (G124L, I127A)

Tags

Flag

Expression

Mammalian

Mutation

G124L I127A

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(cAMP-1.6)/pcDNA3

Plasmid

#53594

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Expression

Mammalian

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

Plasmid

#171435

Purpose

Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

Lifeact-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ecDHFR(DD)-MS2CP-VPg(FCV)

Plasmid

#167313

Purpose

Template pDNA to prepare DD-CaVT mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

DD-CaVT

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

Plasmid

#225244

Purpose

Mammalian expression of iPASnFR-L fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

Plasmid

#69906

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

Plasmid

#114112

Purpose

fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

NanoLuc; Volvox channelrhodopsin 1; EYFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES-mCE NLS

Plasmid

#82466

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K13-15R

Plasmid

#63563

Purpose

Expression of human H2A with K13R and K15R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 13 and Lysine 15 are now Arginine

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-3myc-6His-EZH2 21A

Plasmid

#42663

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21A

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-3myc-6His-EZH2 21D

Plasmid

#42664

Depositor

Mien-Chie Hung

Article

Cha et al Science. 2005 Oct 14;310(5746):306-10.

Insert

EZH2 (EZH2 Human)

Tags

3Myc and 6His

Expression

Mammalian

Mutation

S21D

Available Since

July 17, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag mTOR S2035T D2357E

Plasmid

#26605

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

mTOR (MTOR Human)

Tags

Flag

Expression

Mammalian

Mutation

S2035T D2357E

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

Plasmid

#75203

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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