We narrowed to 6,662 results for: LYC;

1. Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

   Plasmid

   #15602

   Depositor

   Susan Lindquist

   Article

   Tessier et al Nature. 2007 May 31. 447(7144):556-61.

   Insert

   Sup35 NM (SUP35 Budding Yeast, Candida albicans)
   

   Use

   Tags

   7xHis and Cysteine

   Expression

   Bacterial

   Mutation

   4 G/A. Four glycines mutated to alanines. The pos…

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA3790

   Plasmid

   #70110

   Purpose

   mtDNA elimination/production of rho-0 cells

   Depositor

   Mikhail Alexeyev

   Article

   Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

   Inserts

   EGFP
   hUNG1 Y147A
   Neo
   

   Use

   Tags

   None, human OTC mitochondrial targeting sequence,…

   Expression

   Mammalian

   Mutation

   Y147A, aa's 1-77 removed

   Promoter

   CMV, EF1a, and SV40 and native

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-Ub KO.G76V

   Plasmid

   #11932

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin KO.G76V (RBX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Conjugation deficient mutant. All 7 lysines of ub…

   Promoter

   Available since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. GPI-RpHLuorin2

   Plasmid

   #171721

   Purpose

   GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to GPI anchor
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Spike-VLP

   Plasmid

   #173922

   Purpose

   Expresses virus-like particles based upon the original SARS-Cov-2

   Depositor

   Andreas Stuermer

   Inserts

   SARS-2 Spike Protein (S Synthetic, Human, SARS-COV-2)
   SARS-Cov-2 E protein (E Synthetic, Human, SARS-COV-2)
   SARS-Cov-2 M Protein (M Synthetic, Human, SARS-COV-2)
   

   Use

   Tags

   no tags, native protein

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

   Available since

   April 11, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. YIP sHRPa-Aga1P

   Plasmid

   #73151

   Purpose

   Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-Aga1P
   

   Use

   Tags

   V5 tag

   Expression

   Yeast

   Mutation

   Promoter

   glyceraldehyde-3-phosphate dehydrogenase GPD prom…

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PVS10

   Plasmid

   #104398

   Purpose

   polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-HLA-A2_GFP

   Plasmid

   #85161

   Purpose

   HLA.A2_GFP fusion with 2 glycines in between

   Depositor

   Tone Fredsvik Gregers, Sébastien Walchli

   Article

   Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

   Insert

   HLA.A2 (HLA-A Human)
   

   Use

   Gateway entry vector

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-Cdc42(V12)

   Plasmid

   #11399

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Glycine 12 changed to Valine.

   Promoter

   Available since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGTRm

    Plasmid

    #127197

    Purpose

    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA PCR template
    

    Use

    Template plasmid for generation of golden gate pc…

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-YTB

    Plasmid

    #26721

    Depositor

    Edward Callaway

    Article

    Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

    Insert

    venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Rabies virus (strain SAD B19), Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1A

    Plasmid

    #162692

    Purpose

    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOME0182

    Plasmid

    #178403

    Purpose

    Expresses KSHV K8.1 glycoprotein hyp tagged

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    KSHV K8.1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGE476

    Plasmid

    #153267

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK-Cas9-gate

    Plasmid

    #113743

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE-30-RexYFP

    Plasmid

    #141070

    Purpose

    Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    RexYFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed Leucine 169 to Proline, changed Tyrosine …

    Promoter

    T5

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS1422

    Plasmid

    #12548

    Depositor

    Robert Sobol

    Article

    Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

    Insert

    human Methylpurine DNA glycosylase (MPG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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