We narrowed to 66,529 results for: des.1

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LentiV_Neo_POU2F3_CR

Plasmid

#122175

Purpose

Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

3 silent mutations for gRNA resistance (882g-c, 8…

Promoter

EFS

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 K427V

Plasmid

#47892

Purpose

Expresses myc tagged Miro1 K427 constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 K427V (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

K427V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
GFP-NPM NESM

Plasmid

#13282

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn-FLEX.iGABASnFR.F102G

Plasmid

#112164

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 HA pMT2

Plasmid

#58729

Purpose

expression of HA tagged rat Alpha2 delta-1 in mamalian cells

Depositor

Annette Dolphin

Article

Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe puro C-Raf 22W

Plasmid

#12593

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

N-term truncated mut of Raf-1 (RAF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

22W, N-terminally truncated

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pET-21a(+)-AK1-His

Plasmid

#118977

Purpose

Bacterial expression plasmid for Adenylate kinase isoenzyme 1

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

Plasmid

#39455

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CDmu (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136 with mutations H1672 to Y, 1674D to A

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-GCaMP3fast

Plasmid

#74422

Purpose

Express fluorescent calcium indicator GCaMP3fast in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3fast

Expression

Mammalian

Mutation

W4Y D356A

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c2x-MT1

Plasmid

#32101

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

Metallothionein (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
Cav1-mRed

Plasmid

#12681

Depositor

Richard Pagano

Article

Sharma et al Mol Biol Cell. 2004 Jul . 15(7):3114-22.

Insert

canine caveolin-1 (CAV1 Canis familiaris)

Tags

mRed

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
mTLR1

Plasmid

#13080

Depositor

Ruslan Medzhitov

Insert

TLR1 (Tlr1 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
UEV1

Plasmid

#25619

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

UEV1 (UBE2V1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA1 Flag ATXN1[30Q]

Plasmid

#33236

Depositor

Huda Zoghbi

Article

Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

Insert

Ataxin-1 (ATXN1 Human)

Tags

Flag

Expression

Mammalian

Mutation

insert contains a stretch of 29 amino acids (27 Q…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only
pLX304 Flag-mCherry-eEF1A1_IRES-GFP

Plasmid

#198383

Purpose

eEF1A1 fluorescent reporter

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eEF1A1 (EEF1A1 Human)

Use

Lentiviral

Tags

Flag-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAGI1

Plasmid

#23523

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHCAG-L2EOP

Plasmid

#51783

Purpose

The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expression

Depositor

Paula Lam

Article

Sia et al J Gene Med. 2010 Oct;12(10):848-58. doi:

Inserts

Luciferase-2A-EBNA-1
OriP

Use

Herpes simplex virus type-1 amplicon vector

Promoter

CAG

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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EF.deltaBHES1.Ubc.GFP

Plasmid

#24982

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

DNA-binding domain disrupted by E43A, K44A, R47A …

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pOPINO_NbH11-H4

Plasmid

#155364

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

Depositor

Ray Owens

Article

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

H11-H4

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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