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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,929 results for: nin

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Showing: 4101 - 4120 of 66929 results

  3. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Lynda Chin
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  6. ABL1_HUMAN_D0

    Plasmid
    #79727
    Purpose
    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    ABL1 (ABL1 Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  7. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208942-plasmid-map-sequence-id-415667

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  8. pLV-EF1A-CD19.8H.8TM.BB.3z

    Plasmid
    #200671
    Purpose
    Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Publication
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    200671-plasmid-map-sequence-id-391911

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  10. EasyFusion Halo-mAID

    Plasmid
    #112852
    Purpose
    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-mAID
    Use
    Synthetic Biology
    Promoter
    No
    Available since
    July 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112852-plasmid-map-sequence-id-217036

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  11. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198467
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.
    Depositor
    Viviana Risca
    Publication
    Soroczynski et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198467-plasmid-map-sequence-id-390254

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  14. pCMV/3' Box_(GLuc)_TOP1

    Plasmid
    #68434
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68434-plasmid-map-sequence-id-120212

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  15. pMH3

    Plasmid
    #52528
    Purpose
    serves as PCR-template to generate HR donors for C-terminal GFP-tag
    Depositor
    Klaus Foerstemann
    Publication
    Bottcher et al Nucleic Acids Res. 2014 Apr 19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP and Blasticigin resistance cassette
    Use
    Blunt cloning kit
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52528-plasmid-map-sequence-id-81488

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  17. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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Showing: 4101 - 4120 of 66929 results