We narrowed to 6,775 results for: wus

1. EF1a_POU5F1_P2A_Hygro_Barcode

   Plasmid

   #120474

   Purpose

   Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)

   Plasmid

   #105192

   Purpose

   Module 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIG II-BTLA-mGFP

   Plasmid

   #180798

   Purpose

   Expression of mouse BTLA fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   BTLA (Btla Mouse)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   MSCV

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2-actinb-kcnj13-EGFP

   Plasmid

   #164940

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Expression

   Mutation

   Stop codon deleted

   Promoter

   actinb (actb2) promoter

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

   Plasmid

   #105209

   Purpose

   Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS170-enNTS1

   Plasmid

   #199152

   Purpose

   JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

   Depositor

   Daniel Scott

   Article

   Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

   Insert

   enNTS1 (Ntsr1 Rat)
   

   Use

   Tags

   10xHis tag, Avi tag, MBP, and muGFP

   Expression

   Bacterial

   Mutation

   V208M, C273S, C391S and C393S (over NTS1-B5)

   Promoter

   Lac/T5

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 NLRP1 P1214R

   Plasmid

   #166822

   Purpose

   Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   P1214R

   Promoter

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hA1-BE3

   Plasmid

   #113418

   Purpose

   Expresses hA1-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA1-BE3 (APOBEC1 Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. double_sgRNA targeting e3 and e7

   Plasmid

   #190689

   Purpose

   sgRNAs targeting enhancer 3 and 7 of MYC separately

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNAs targeting enhancer 3 and 7 of MYC separately
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_Hygro(+)_GRHL1-L3F6H

    Plasmid

    #177765

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RA

    Plasmid

    #191684

    Purpose

    For knocking p2a-mNeonGreen into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

    Plasmid

    #191688

    Purpose

    For knocking p2a-EFGP into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

    Plasmid

    #170217

    Purpose

    Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CASP8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C360A

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA2880_pPB-TREtight-FLAG-Esrrb-InO

    Plasmid

    #124178

    Purpose

    piggyBac-based Dox-inducible expression of FLAG-Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Promoter

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pAAVsc.U6-sgFah.CMV/CB-EGFP

    Plasmid

    #121508

    Purpose

    Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

    Plasmid

    #170225

    Purpose

    Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    ER (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Y537S

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiV_Neo_SIK3_CR_T142Q

    Plasmid

    #108109

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-cAICDA-BE3

    Plasmid

    #113422

    Purpose

    Expresses cAICDA-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE_puro_ZFP64_N_3XFLAG

    Plasmid

    #118693

    Purpose

    Express 3xflag tagged ZFP64 for flag ChIP experiments

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    ZFP64 (ZFP64 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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