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Showing: 6181 - 6210 of 8427 results

  7. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50593-plasmid-map-sequence-id-451910

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  8. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid
    #165424
    Purpose
    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165424-plasmid-map-sequence-id-331265

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  9. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

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  10. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Jie Na
    Article
    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/
    Insert
    dCas9-VPR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    89985-plasmid-map-sequence-id-180638

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  16. groSL-GFP

    Plasmid
    #109389
    Purpose
    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pgroSL-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109389-plasmid-map-sequence-id-210771

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  17. pDIV643

    Plasmid
    #226182
    Purpose
    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi
    Insert
    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TEF1p
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226182-plasmid-map-sequence-id-449156

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  19. ibpAB-GFP

    Plasmid
    #109390
    Purpose
    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pibpAB-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109390-plasmid-map-sequence-id-210782

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  22. htpG1-GFP

    Plasmid
    #109387
    Purpose
    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG1-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109387-plasmid-map-sequence-id-210728

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  23. pCBC-MT2T3

    Plasmid
    #50594
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50594-plasmid-map-sequence-id-75674

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  25. htpG2-GFP

    Plasmid
    #109388
    Purpose
    GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    phtpG2-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109388-plasmid-map-sequence-id-210731

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  27. pJAT11

    Plasmid
    #204290
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204290-plasmid-map-sequence-id-405507

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  28. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Insert
    Cas3
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229538-plasmid-map-sequence-id-459007

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  30. pCP-tRNA

    Plasmid
    #133812
    Purpose
    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis
    Depositor
    Geraldine Butler
    Article
    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001
    Insert
    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133812-plasmid-map-sequence-id-263618

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Showing: 6181 - 6210 of 8427 results