We narrowed to 16,619 results for: puromycin

1. pUC19-EAMP

   Plasmid

   #138265

   Purpose

   mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   iCas9 target-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a-HTLV

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-FLAG-RHEB-IRES-Puro

   Plasmid

   #120576

   Purpose

   Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   RHEB (RHEB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. nSR100L4

   Plasmid

   #35174

   Depositor

   Benjamin Blencowe

   Article

   Calarco et al Cell. 2009 Sep 4;138(5):898-910.

   Insert

   MmnSR100
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMSCV-puro-mMcl-1 KR

   Plasmid

   #32981

   Depositor

   Joseph Opferman

   Article

   Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

   Insert

   Mcl-1 (Mcl1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   All Lys to Arg

   Promoter

   LTR

   Available Since

   March 15, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pLEX-FHH-Raf1RBD

   Plasmid

   #120707

   Purpose

   Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Raf1 RBD
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Raf1 amino acids 1-149

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Degron-KI PIK3CA donor vector

   Plasmid

   #65621

   Purpose

   Donor vector to generate Degron-KI DD-PIK3CA

   Depositor

   Frank Stegmeier

   Article

   Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

   Insert

   PIK3CA Degron-KI left and right HR arms
   

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCF226

   Plasmid

   #121667

   Purpose

   EFS-Cas9-wt-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   cas9
   

   Use

   Lentiviral

   Promoter

   EF-1a core promoter

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - C3orf17 sgRNA 1

   Plasmid

   #70652

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBRIT Pax7 34C

   Plasmid

   #17522

   Depositor

   Michael Rudnicki

   Article

   McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

   Insert

   Pax7 (Pax7 Mouse)
   

   Use

   Cre/Lox and Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-33

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pSMP-SMYD2_3

    Plasmid

    #36351

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SMYD2 (SMYD2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

11. JL302: pMVP/Puro-DEST

    Plasmid

    #121856

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ1653_sgSOD1

    Plasmid

    #63711

    Purpose

    Express sgSOD1 in mammalian cells of human.

    Depositor

    Stanley Qi

    Article

    Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

    Insert

    sgSOD1, CAG promoter, mCherry, puro
    

    Expression

    Mammalian

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid

    #70655

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C9orf114
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. lentiCRISPR - C9orf114 sgRNA 2

    Plasmid

    #70654

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against C9orf114
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. MSCV-6NBC-GFP-PGK-Puro-Barcode83

    Plasmid

    #83760

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode83
    

    Use

    Retroviral

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBRIT Pax7 N33-170C

    Plasmid

    #17526

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of aa 34-159 (paired domain)

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pBRIT Pax7 N215-273C

    Plasmid

    #17527

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Insert

    Pax7 (Pax7 Mouse)
    

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of aa 216-272 (homeodomain)

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pQCXIP X2/pTER shLUC (w454-1)

    Plasmid

    #17491

    Purpose

    Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase shRNA
    

    Use

    RNAi and Retroviral

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenCMV-PMMars1CyGFP

    Plasmid

    #78315

    Purpose

    Lentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning protein

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    Mars1Cy-TMD-GFP
    

    Use

    Lentiviral

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. shCDK6(2)

    Plasmid

    #73553

    Purpose

    shRNA(2) against CDK6

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK6
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only