We narrowed to 7,368 results for: actin
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Plasmid#122957PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expressionDepositorAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only
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Double UP sfGFP to mScarlet
Plasmid#120261PurposeNatively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.DepositorInsertsfGFP/mScarletP2A-mcs
ExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector
Plasmid#114430PurposeTarget a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locusDepositorInsertGCaMP6s, tdTomato, tTA2
UseCre/Lox and Mouse TargetingPromoterTRE2, CAGAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184V
Plasmid#106187PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVMutationGltI: A184VPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG-FLEX.iGABASnFR.F102G
Plasmid#112167PurposeGABA SensorDepositorInsertiGABASnFR.F102G
UseAAVMutationF102GPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-eGtACR1-mRuby2-ST
Plasmid#109136PurposeAnion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteGtACR1-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-C1V1-TT-mRuby2-ST
Plasmid#109124PurposeCation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertC1V1-TT-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mGSDMD1-251
Plasmid#111565PurposeExpress ELANE-cleaved mouse GSDMD in mammalian cellsDepositorAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM705 Gst (Cys)
Plasmid#109376PurposeGsX chimera with Gαt C-terminus (Pertussis toxin sensitive)DepositorInsertGst chimera
TagsNoneExpressionMammalianMutationCys at position 391 (Pertussis Toxin Sensitive)PromoterCAGAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-Venus-iGluSnFR.S72A
Plasmid#106203PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
Plasmid#105922PurposeAAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITRDepositorInsertsmKate2
IRES-Multiple cloning site
UseAAVMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCAGGS-hPhyB621-mCherry-HRasCT
Plasmid#100282PurposeExpresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cellsDepositorInsertPhytochrome B (1-621 aa) (PHYB Mustard Weed)
TagsmCherry fused with HRas C-terminus for plasma mem…ExpressionMammalianMutationHuman codon optimized PhyB (1-621 aa)PromoterCAG promoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SaRFN
Plasmid#85755PurposeExpresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-SadCas9
UseCRISPRTags3xHA tag, Csy4, and FokI fusion via 36 amino acid…ExpressionMammalianMutationD10A, N580APromoterCAGAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Tfe3-pgk-hph
Plasmid#48755PurposePiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selectionDepositorAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Tfe3-pgk-hph
Plasmid#48755PurposePiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selectionDepositorAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo
Plasmid#74919PurposeMammalian expression of mKlf2 and mNanog-mCherryDepositorTagsT2A and mCherryExpressionMammalianPromoterCAGAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18 dSAP
Plasmid#68830PurposeExpresses c-myc-tagged human RAD18 deleting SAP domainDepositorInsertc-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
Tagsc-mycExpressionMammalianPromoterCAGAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only