We narrowed to 9,001 results for: ef1;

1. pEFBOS_CreIRESpuro

   Plasmid

   #183812

   Purpose

   Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

   Depositor

   Ed Stanley

   Article

   Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-mAmetrine-CAAX

   Plasmid

   #216132

   Purpose

   Lentiviral vector for expressing mAmetrine localizing to the plasma membrane.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-mAmetrine-CAAX-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSBbi_RP_3XNLS-MicroID2-FLAG

   Plasmid

   #188523

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   3XNLS-MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132398

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFC333

   Plasmid

   #87844

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   ble (bleomycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. alpha7nACHR_Nacho_GCAMP7s

   Plasmid

   #182940

   Purpose

   alpha7 nACHR expression in mammalian cell lines with calcium reporter

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBA409

   Plasmid

   #85971

   Purpose

   EF1a-sfGFP-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sfGFP
   Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF.DEST51-VPS35-mScarlet

    Plasmid

    #215474

    Purpose

    Expression vector with VPS35 tagged with mScarlet

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    VPS35 (VPS35 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti_rtTA-P2A-BFP_HygroR

    Plasmid

    #186738

    Purpose

    rtTA-P2A-BFP expression construct cloned into lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    rtTA
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-FoxO1-Clover

    Plasmid

    #67759

    Purpose

    Fluorescent fusion protein for tracking Akt signaling

    Depositor

    Peter Rotwein

    Article

    Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

    Insert

    Fox01 (Foxo1 Mouse)
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mutation

    Changed Serine 209 to Alanine, changed Histidine …

    Promoter

    EF1a

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-Bos TRIF Flag

    Plasmid

    #41550

    Depositor

    Tom Maniatis, Kate Fitzgerald

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    Trif (TICAM1 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

15. LLP791_PB_dCas9-SSSavi_BirA

    Plasmid

    #211786

    Purpose

    PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pCAGG abd pEF1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-EFS-PCP-3xSNAP-NLS

    Plasmid

    #236388

    Purpose

    Overexpression of PCP-3xSNAP in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    PCP-3xSNAP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSimple-miR302/367

    Plasmid

    #98748

    Purpose

    Episomal reprogramming

    Depositor

    James Thomson, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

    Insert

    miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCP-ADAR2-DD(E488Q)

    Plasmid

    #170125

    Purpose

    Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD(E488Q)-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-NLS-mTagBFP2

    Plasmid

    #216128

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid

    #188524

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    2XMTS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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