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Plasmid#182844Purposemutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG inductionDepositorInsertTRX (w Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-HseIF4E_AF
Plasmid#148669PurposeBacterial Expression of HseIF4EDepositorInsertHseIF4E (EIF4E Human)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence of i…PromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996
Plasmid#171131PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluSz 3501-4065
Plasmid#171132PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluY WT 2598-2996
Plasmid#171134PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066
Plasmid#171136PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPC2(E643A)-mCherry
Plasmid#135187PurposeMutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of glutamate 643 to alaninePromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIE-N-hα2M-H6
Plasmid#128493PurposeExpresses human N-α2M (from S24 to E908) in insect cells. With C-t H6x tag.DepositorInsertA2M (S24-E908) (A2M Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS6-N-hα2M-H6
Plasmid#128496PurposeExpresses human N-α2M (from G27 to A788) in mammalian cells. With C-t H6x tag.DepositorInsertA2M (G27-A788) (A2M Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD52-bglpA
Plasmid#89704PurposeHuman CD52 expression plasmid with short polyADepositorInsertCMV-CD52-bglpA (CD52 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_nmPKA-CαN-mPAGFP
Plasmid#114130PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP, with G2A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321A
Plasmid#115339PurposeMammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:GUS
Plasmid#108444PurposeArabidopsis transformation, expresses HDT2 promoter/GUS fusion to study expression pattern in rootsDepositorInsertHDT2 promoter (HD2B Mustard Weed)
UseTagsGUSExpressionPlantMutationPromoterHDT2Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT2:HDT2-GFP
Plasmid#108566PurposeArabidopsis transformation, expresses HDT2/GFP fusion with HDT2 promoter to study expression pattern in rootsDepositorInsertHDT2 (HD2B Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT2Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500
Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIR-192-Reporter
Plasmid#71872PurposeMammalian expression vector for the analysis of miR-19α activityDepositorInsertReverse complementary sequence of miR-192
UseTagsfirefly luciferaseExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only