We narrowed to 7,375 results for: ef1a

1. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-EF1a-MegaGate-DD-Hygro

   Plasmid

   #175267

   Purpose

   MegaGate destination with C-terminal DD tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   Destabilization Domain

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-DIO-mCherry

   Plasmid

   #50462

   Purpose

   Double floxed mCherry under the control of EF1a promoter

   Depositor

   Bryan Roth

   Insert

   mCherry
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV x EF1a_EKAREN5-ires-puro

   Plasmid

   #167821

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-H2B-miRFP670

   Plasmid

   #212668

   Purpose

   Express tagged H2B

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   miRFP670

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Ef1a-InteinC-CreC

   Plasmid

   #187615

   Purpose

   AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

   Depositor

   Il Hwan Kim

   Article

   Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

   Insert

   InteinC-CreC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Ef1a-CreN-InteinN

   Plasmid

   #187614

   Purpose

   AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

   Depositor

   Il Hwan Kim

   Article

   Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

   Insert

   CreN-InteinN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_EF1a-DjCas13d-msfGFP-Blast

   Plasmid

   #226008

   Purpose

   DjCas13d protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   DjCas13d RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLENTI EF1alpha FLAG/HA-PALI1

    Plasmid

    #114445

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    PALI1 (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1aH-PIK3CA H1047R

    Plasmid

    #180031

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    EF1a

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAID1.2-EF1a-CGFP-mAID

    Plasmid

    #140608

    Purpose

    Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSmart-EF1a-Nla-cas3

    Plasmid

    #178881

    Purpose

    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas3c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-FLEX-GTB

    Plasmid

    #26197

    Depositor

    Edward Callaway

    Article

    Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

    Insert

    Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mutation

    TVA, rabies glycoprotein are mammalian codon opti…

    Promoter

    Available Since

    Nov. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAIDFA-EF1a-NmScarlet-mAID

    Plasmid

    #140615

    Purpose

    Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1 mutated F74A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

    Plasmid

    #204621

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

    Plasmid

    #192651

    Purpose

    3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    dCas9-10xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEnt L1L3 EF1a-tTA-2

    Plasmid

    #26803

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Elongation Factor 1 alpha promoter and tTA transgene
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB-EF1a-NLuc-IRES-Puro

    Plasmid

    #130936

    Purpose

    PiggyBac vector for constitutive NanoLuc expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Ef1a-DIO_EGFP-GFE3

    Plasmid

    #79871

    Purpose

    For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAF0036 Ef1a-Si74-2a-EGFP

    Plasmid

    #193463

    Purpose

    Expression of Si74 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LV-Sox10MCS5-GFP-EF1a-mCherry

    Plasmid

    #115782

    Purpose

    Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Inserts

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    EF1alpha promoter driven constitutive mCherry
    

    Use

    Lentiviral

    Tags

    EF1α promoter fused to mCherry and cfos promoter …

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-EF1a-ArgiNLS-EGFP

    Plasmid

    #220594

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-EF1a-DIO-mGreenLantern

    Plasmid

    #164468

    Purpose

    Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    EF1a

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV-EF1a-ArgiNLS-mGreenLantern

    Plasmid

    #220596

    Purpose

    Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT3-EF1aH Yap S127A

    Plasmid

    #86497

    Purpose

    Mammalian expression of FLAG-tagged Yap containing S127A mutation

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes associated protein 1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLenti-EF1a-CD90.2-Rluc_miR

    Plasmid

    #163360

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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