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We narrowed to 560 results for: DHFR

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Showing: 401 - 420 of 560 results

  2. 2xFKBP-smFP(myc)-KDEL

    Plasmid
    #173021
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
    Depositor
    Matthew Kennedy
    Publication
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xFKBP-smFP(myc)-KDEL
    Tags
    FKBP and smFP(myc) non-fluorescent
    Expression
    Mammalian
    Available since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173021-plasmid-map-sequence-id-339210

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  3. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102886-plasmid-map-sequence-id-194712

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  4. pMVP and pMAGIC Cloning Systems

    Plasmid kit
    #1000000155
    Purpose
    For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC).
    Depositor
    Christopher Newgard
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Adenoviral, Lentiviral, Mammalian Expression
    Editing type
    CRISPR
    Cloning type
    MultiSite Gateway
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  5. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Publication
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Expression
    Yeast
    Mutation
    DHFR P66L
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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  6. pCSJ982

    Plasmid
    #176622
    Purpose
    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Publication
    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11
    Insert
    Ub-MPGLW-Fbp1
    Tags
    Ubiquitin N-terminal fusion and triple flag tag C…
    Expression
    Yeast
    Mutation
    Changed first 4 N-terminal amino acid residues of…
    Promoter
    TDH3tc3
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    176622-plasmid-map-sequence-id-356527

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  19. pMT2 Beta1b GFP

    Plasmid
    #89893
    Purpose
    Mammalian expression of Beta1b GFP
    Depositor
    Annette Dolphin
    Publication
    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cacnb1 (Cacnb1 Rat)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    GFP sequence inserted just before the Stop codon
    Promoter
    adenovirus major late promoter
    Available since
    April 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89893-plasmid-map-sequence-id-176909

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Showing: 401 - 420 of 560 results