We narrowed to 1,577 results for: MK

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MAC-DPP4

Plasmid

#172416

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Dipeptidyl peptidase 4 (DPP4 Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z23

Plasmid

#120066

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

Plasmid

#204694

Purpose

This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

BRD4-NUT (BRD4, NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

direct fusion of BRD4 and NUT

Promoter

CMV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD3 (OTU+UBA, aa 52-275)

Plasmid

#61411

Purpose

Expresses human OTUD3 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 276-398.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5

Plasmid

#157804

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-12-C, 21-G

Plasmid

#124683

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBR322 hCDK4 promoter

Plasmid

#16571

Depositor

Bert Vogelstein

Article

Hermeking et al Proc Natl Acad Sci U S A. 2000 Feb 29. 9

Insert

CDK4 promoter (CDK4 Human)

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZc-HsACTB*-thymosinB-8His

Plasmid

#111146

Purpose

Expresses human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration

Tags

Thymosin beta and a His-tag

Expression

Yeast

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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SNAP-Paxillin

Plasmid

#186143

Purpose

For expresseion of recombinant paxillin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

GST

Expression

Bacterial

Mutation

Paxillin C1997A (silent mutation for cloning)

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ400

Plasmid

#177269

Purpose

USER cloning-compatible 2μ vector (URA3)

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEDJ437

Plasmid

#177270

Purpose

USER cloning-compatible 2μ vector (HIS3)

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-Map7-1333-end

Plasmid

#46070

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-1333-end (Map7 Mouse)

Expression

Bacterial

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(rInsp)-Ins-GLuc

Plasmid

#89928

Purpose

Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

Depositor

Melanie Cobb

Article

Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

Insert

human insulin-Gaussia-Luciferase (INS Synthetic, Human)

Expression

Mammalian

Mutation

DNA sequence for Gaussia luciferase was humanized…

Promoter

rat Insulin II promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z07

Plasmid

#124790

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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