We narrowed to 7,394 results for: ef1a;

1. pSicoR-Ef1a-mCh

   Plasmid

   #31847

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. PEW-pEF1A-inactEGFP

   Plasmid

   #212009

   Purpose

   Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

   Depositor

   Catherine Wu

   Article

   Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

   Insert

   Inactivated eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHIV-EF1a-2A-ZsGreen

   Plasmid

   #231985

   Purpose

   Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   2A-ZsGreen
   

   Use

   Lentiviral

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_EF1a-->GssmCherry-PNE

   Plasmid

   #162157

   Purpose

   Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 EF1a-EGFP

   Plasmid

   #231925

   Purpose

   3rd generation lentiviral transfer plasmid with EGFP reporter gene

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   EF-1a

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Expression

   Mammalian

   Promoter

   hU6, EF1a

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-V5-Bet1

   Plasmid

   #120243

   Purpose

   Expresses V5-tagged Bet1 in lentiviral vector

   Depositor

   Larry Gerace

   Insert

   Bet1 (Bet1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE2-EF1aL-mCherry-W

   Plasmid

   #126703

   Purpose

   Constitutive, ubiquitous mCherry expression.

   Depositor

   Darrell Kotton

   Article

   McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

   Insert

   mCherry
   

   Use

   Lentiviral

   Promoter

   Ef1aL

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-EF1aL-dsRed-W

    Plasmid

    #195863

    Purpose

    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

    Depositor

    Darrell Kotton

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed

    Promoter

    EF1aL

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV-EF1aGFP2AKAT7(E508Q)-W

    Plasmid

    #159293

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 E508Q
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

    Promoter

    human EF1a promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EF1a-LIC-V5

    Plasmid

    #120248

    Purpose

    For LIC cloning with C-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVH21-Tier1-PhEF1a

    Plasmid

    #169561

    Purpose

    Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112924

    Purpose

    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Piggybac transposon vector

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1a-Usp9x_CD-mCherry

    Plasmid

    #170380

    Purpose

    Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

    Depositor

    Miguel Ramalho-Santos

    Article

    Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

    Insert

    Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
    

    Tags

    mCherry-3xFlag

    Expression

    Mammalian

    Mutation

    Catalytic domain

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3-EF1A-CCNE2

    Plasmid

    #162554

    Purpose

    Overexpression of CCNE2

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE2 (CCNE2 Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mc2 mNeon EF1a

    Plasmid

    #206224

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-sCre

    Plasmid

    #55635

    Purpose

    Expresses sCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV-EF1a-mCherryCXCR4-W

    Plasmid

    #200102

    Purpose

    Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR and Lentiviral

    Mutation

    silent mutations (c.180C>T, 438G>A, 495C>…

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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