We narrowed to 5,035 results for: mcs

1. Hy_pMT dati MCS Exon

   Plasmid

   #110119

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   dati (dati Fly)
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT dati MCS circfirefly_1100

   Plasmid

   #110117

   Purpose

   Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT dati MCS circfirefly_900

   Plasmid

   #110116

   Purpose

   Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hy_pMT dati MCS circfirefly_700

   Plasmid

   #110115

   Purpose

   Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hy_pMT dati MCS circfirefly_500

   Plasmid

   #110114

   Purpose

   Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CBh-mKate2-IRES-MCS (WT-WT)

   Plasmid

   #105921

   Purpose

   AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

   Depositor

   Marcella Patrick

   Has Service

   AAV2

   Inserts

   mKate2
   IRES-Multiple cloning site
   

   Use

   AAV

   Mutation

   codon optimized

   Promoter

   CBh and same CBh promoter as mKate2

   Available Since

   Feb. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. AAV-GfaABC1D-MCS--4x6T-WPRE

   Plasmid

   #196417

   Purpose

   Empty vector for astrocyte-selective expression in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Type

   Empty backbone

   Use

   AAV; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plenti-CMV-MCS-RFP-SV-puro

   Plasmid

   #109377

   Purpose

   This plasmid expresses dsRed2 and puromycin resistance

   Depositor

   Behzad Gerami-Naini, Jonathan Garlick

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   dsRed2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

   Plasmid

   #138152

   Purpose

   Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   eGFP-MCS
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCS-TurboID MSCV-IRES-GFP

    Plasmid

    #207957

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TurboID-MCS MSCV-IRES-GFP

    Plasmid

    #207958

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-EGFP-eDHFR(69K6)-MCS

    Plasmid

    #172100

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    EGFP-eDHFR(69K6)-20aa
    

    Expression

    Mammalian

    Mutation

    eDHFR(69K6): Hexalysine (K6) sequence is inserted…

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

    Plasmid

    #69908

    Purpose

    Expresses a GFP circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

    Plasmid

    #69901

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA

    Plasmid

    #110203

    Purpose

    Recipient vector for Cranial Neural Crest-specific expression of protein of interest

    Depositor

    Tatjana Sauka-Spengler

    Insert

    enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
    

    Tags

    eGFP

    Expression

    Bacterial

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK231 (AAVS1 CMV-MCS-PURO)

    Plasmid

    #105924

    Purpose

    Donor for integration at the human AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SPDK3876(TRV-RNA2-pPEBV-MCS)

    Plasmid

    #149275

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter
    

    Use

    T-dna vector

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-TgKI-MCS-tdTomato-polyA

    Plasmid

    #174022

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    tdTomato
    

    Use

    Zebrafish knock-in tagging

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hygro-TRE>{MCS}:T2A:EGFP

    Plasmid

    #236250

    Purpose

    Hygromycin selected lentiviral vector with MCS for tetracycline-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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