We narrowed to 9,662 results for: try

1. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

   Plasmid

   #105803

   Purpose

   Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-FLAG; ORF2: TEV-mClover3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBabe-kappa-HA-dL5-myc-ADRB2

   Plasmid

   #101253

   Purpose

   Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)

   Depositor

   Marcel Bruchez

   Article

   Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.

   Insert

   kappa-HA-dL5-myc-ADRB2 (ADRB2 Human, Budding Yeast)
   

   Use

   Retroviral

   Tags

   FAP-dL5 and HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   MMLV LTR

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

   Plasmid

   #153956

   Purpose

   Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   June 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDONR223 SARS-CoV-2 ORF7B C-trunc

   Plasmid

   #153957

   Purpose

   Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   June 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

   Plasmid

   #105806

   Purpose

   Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   ORF1: 3C-Cerulean; ORF2: TEV-Amber

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

   Plasmid

   #240865

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

   Plasmid

   #240094

   Purpose

   GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

   Depositor

   Mark Verheijen

   Article

   Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

   Insert

   U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
   

   Use

   AAV and CRISPR

   Tags

   SaCas9 + 3X HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA

   Plasmid

   #239766

   Purpose

   Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   K1425TAG, Y371C

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA

    Plasmid

    #239767

    Purpose

    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K1546TAG, Y371C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-P2A-eGFP

    Plasmid

    #239769

    Purpose

    Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and eGFP in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K1435TAG, Y371C

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C, I1652N)-HA

    Plasmid

    #239770

    Purpose

    Expresses pathogenic (I1652N) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K1425TAG, Y371C, I1652N

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , I1652N)-HA

    Plasmid

    #239771

    Purpose

    Expresses pathogenic (I1652N) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    I1652N, Y371C, K1546TAG

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C, T1785P)-HA

    Plasmid

    #239772

    Purpose

    Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K1425TAG, Y371C, T1785P,

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C , T1785P)-HA

    Plasmid

    #239773

    Purpose

    Expresses pathogenic (T1785P) TTX-resistant variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

    Insert

    Scn8a (Scn8a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    K1546TAG, Y371C, T1785P

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLCKO-CMV-TMPRSS2-IRES-Hygromycin

    Plasmid

    #237477

    Purpose

    Encodes full length human TMPRSS2 protein, along with hygromycin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    IRES-Hygromycin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

    Plasmid

    #238132

    Purpose

    This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMEM106B
    

    Use

    Lentiviral

    Tags

    Blasticidin-P2A-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

    Plasmid

    #238131

    Purpose

    This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    TMEM106B
    

    Use

    Lentiviral

    Tags

    Blasticidin-P2A-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR2b-ITGB1(YYFF)-mRuby2

    Plasmid

    #215447

    Purpose

    Gateway entry vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Gateway entry vector

    Tags

    mRuby2

    Expression

    Mutation

    Mutations in the NPxY sites Y783F, Y795F; Silent …

    Promoter

    none

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCR8/GW_TOPO_Cdk13_Untagged

    Plasmid

    #127342

    Purpose

    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

    Insert

    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Expression

    Mutation

    Base pairs 1-1554 of transgene are codon optimize…

    Promoter

    None

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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