We narrowed to 13,748 results for: TIM;

1. MLKL gRNA (BRDN0001149268)

   Plasmid

   #77541

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MLKL

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MLKL (MLKL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. non-targeting control gRNA (BRDN0001487130)

   Plasmid

   #80262

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. non-targeting control gRNA (BRDN0001148500)

   Plasmid

   #80260

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTU422

   Plasmid

   #178982

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Inserts

   gRAMP
   CRISPR array
   

   Use

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. INCTbiosyn-pUB-HspINTBxb1

   Plasmid

   #127519

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase Bxb1.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase Bxb1 coding sequence codon optimized for H. sapiens expression.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. SspB-HaloTag-DHPH-TIAM1

   Plasmid

   #176114

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

   Plasmid

   #109048

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Has Service

   AAV9

   Insert

   eGtACR1-ST
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183536

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

   Insert

   ChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   CaMKIIa

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PEA1

   Plasmid

   #176527

   Purpose

   Delivers all prime editing nickase components in a single plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CbH for Cas9, hU6 for gRNAs

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. non-targeting control gRNA (BRDN0001145598)

    Plasmid

    #80173

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYNL-C1_Rab11a

    Plasmid

    #65705

    Purpose

    Expresses YNL-Rab11a in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Rab11a (Rab11a Mouse)
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ1955

    Plasmid

    #84830

    Purpose

    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized tdTomato
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(ICOS)

    Plasmid

    #177903

    Purpose

    Bacterial expression of his10-tagged human ICOS Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    ICOS (aa 162-199 only) (ICOS Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NUAK1 gRNA (BRDN0001162598)

    Plasmid

    #75577

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #68719

    Purpose

    Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Tobias Bonhoeffer, Tobias Rose, Mark Huebener

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Has Service

    AAV1

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG-FLEX

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2/NS5-YFP-NLS-mut

    Plasmid

    #120832

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5-eYFP "NLS-mut"
    

    Use

    Tags

    eYFP-V5

    Expression

    Mammalian

    Mutation

    R372A/Q373A/K387A/H388A/K389A

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. NUAK1 gRNA (BRDN0001147733)

    Plasmid

    #75576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge