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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

Plasmid

#47580

Purpose

Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Inserts

MKK4-EE (MAP2K4 Human)
MKK7a1-EE (Map2k7 Mouse)

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

Plasmid

#216733

Purpose

Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase and sgCTG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 S310Y

Plasmid

#40992

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

S310Y

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 G309E

Plasmid

#40990

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G309E

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 C311R

Plasmid

#40980

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C311R

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 C311S

Plasmid

#40985

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C311S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 D845A

Plasmid

#40984

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D845A

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 E321G

Plasmid

#40981

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E321G

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pLEX303 TagRFP-T LGALS3 R186S

Plasmid

#202428

Purpose

Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

TagRFP

Mutation

R186S

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 C334S

Plasmid

#40983

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C334S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

Plasmid

#162878

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 C315S

Plasmid

#40986

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C315S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 C338S

Plasmid

#40987

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C338S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ERBB2 C331S

Plasmid

#40989

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C331S

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-GSDMD_1-270

Plasmid

#218229

Purpose

Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 1-270

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_29-484

Plasmid

#218231

Purpose

Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 29-484

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_29-270

Plasmid

#218232

Purpose

Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD (GSDMD Human)

Tags

flag

Expression

Mammalian

Mutation

construct only codes for amino acids 29-270

Available Since

July 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187287

Purpose

Express pmCherry-IL2-AH-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-2A-RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

Plasmid

#187292

Purpose

Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2, RhoA Q63L

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 UBE2C guide 1

Plasmid

#117068

Purpose

single guide RNA targeting UBE2C; guide 1

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

UBCH10 (UBE2C Human)

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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