We narrowed to 6,705 results for: hwa

1. pEANHISECArdB

   Plasmid

   #96967

   Purpose

   Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   E. coli ArdB
   

   Tags

   N-ter TEV cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pClneoMyc-LATS1

   Plasmid

   #66851

   Purpose

   Express Myc-tagged LATS1 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   LATS1 (LATS1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P44L mutation in LATS1 compared to GenBank refere…

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIGAhRC

   Plasmid

   #112510

   Purpose

   human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter

   Depositor

   Charles Miller

   Article

   Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

   Insert

   HIF1B, AhR (ARNT, AHR Budding Yeast, Human)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Gal1,10

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT/TO/HIS/FLAG/HA-ALKBH5

   Plasmid

   #38073

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
   

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-sgH11-eCas9-T2A-BlastR

   Plasmid

   #172492

   Purpose

   For CRISPR-assisted HDR, Hipp11 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TGFbetaR1 (T204D)-pcw107-V5

   Plasmid

   #64629

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   TGFBR1 (transcript variant 1) (TGFBR1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   T204D

   Promoter

   PGK

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMXs-hs-EGR1

   Plasmid

   #52724

   Purpose

   retroviral expression of human EGR1

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLHCX-ATF4 mScarlet NLS

   Plasmid

   #115970

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Human, Synthetic)
   

   Use

   Retroviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-UbC-Blast-2A-STING-mNeonGreen

   Plasmid

   #227185

   Purpose

   Lentiviral expression plasmid encoding STING-mNeonGreen under UbC promoter

   Depositor

   Nir Hacohen

   Article

   Gentili et al Nat Commun. 2023 Feb 4;14(1):611. doi: 1

   Insert

   STING1 (STING1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 IGF1R-BirA(R118G)-HA

    Plasmid

    #109232

    Purpose

    This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

    Depositor

    Victor Dzau

    Article

    Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

    Insert

    IGF1R (IGF1R Human)
    

    Tags

    BirA(R118G) (highly promiscuous form) and HA

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPC1-hVAP-A KD/MD

    Plasmid

    #104449

    Purpose

    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed K94 to D and M96 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT/TO/HIS/FLAG/HA-HNRNPU

    Plasmid

    #38068

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS Donor mClover-LMNA

    Plasmid

    #98970

    Purpose

    Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1a_Flag_TP53_R248W

    Plasmid

    #183114

    Purpose

    Expresses flag-tagged p53 with R248W mutation in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 248 to tryptophan

    Promoter

    EF1a

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HSPB1

    Plasmid

    #155670

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    HSPB1 (HSPB1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

    Plasmid

    #113160

    Purpose

    A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-CMV-6xHis-SUMO1

    Plasmid

    #164939

    Purpose

    Expresses 6xHis tagged human Small Ubiquitin Like Modifier 1 (SUMO1) wild type cDNA

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Small Ubiquitin Like Modifier 1 (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    6XHIS

    Promoter

    CMV

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSecTaq2-hProS-Myc-6xHis

    Plasmid

    #226524

    Purpose

    Expresses TAM Receptor Ligand human PROS1

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

    Insert

    Human PROS1 (PROS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

    Plasmid

    #223965

    Purpose

    Dual fluorescent reporter for histone H2B and for Cdt1

    Depositor

    Hee Won Yang

    Article

    Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

    Insert

    H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670 and mCerulean

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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