We narrowed to 13,748 results for: TIM;

1. NUAK1 gRNA (BRDN0001162525)

   Plasmid

   #75578

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NUAK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NUAK1 (NUAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA13-pHR-SFFV-HaloTag-FBL

   Plasmid

   #199444

   Purpose

   Expression of HaloTag-FBL in mammalian cells

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   FBL (FBL Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

   Plasmid

   #98930

   Purpose

   AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCX-CD28 WT

   Plasmid

   #223640

   Purpose

   Retroviral expression of mouse CD28 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (Cd28 Mouse)
   

   Use

   Retroviral; mammalian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CD80-mGFP

   Plasmid

   #223591

   Purpose

   Lentiviral expression of human CD80 with mGFP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD80 (CD80 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PHKG1 gRNA (BRDN0001144981)

   Plasmid

   #76356

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PHKG1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PHKG1 (PHKG1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB2-mRuby3 Addgene

   Plasmid

   #104005

   Purpose

   Plasmid encoding mRuby3 Addgene

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRuby3 Addgene
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

   Plasmid

   #160140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(nucleoplasmin)-3xHA-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-4mtD3cpv

   Plasmid

   #181874

   Purpose

   Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   4mtD3cpv
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   the 4mtD3cpv CDS differs from the recorded GeneBa…

   Promoter

   CaMKIIa

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG-VP16–RAR-alpha

    Plasmid

    #80434

    Purpose

    Expression of constitutively active human RAR-alpha in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Retinoic acid receptor alpha (RARA Human)
    

    Use

    Tags

    EGFP and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SspB-iRFP-DHPH-TIAM1

    Plasmid

    #176118

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKN3 gRNA (BRDN0001146586)

    Plasmid

    #77947

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.hSyn1.Twitch2B.WPRE.SV40

    Plasmid

    #100040

    Purpose

    AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Has Service

    AAV1

    Insert

    Twitch2B
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVU-coHPDL WT

    Plasmid

    #174128

    Purpose

    Expresses wild type codon-optimized HPDL in mammalian cells

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRY2olig-mCh-Nck

    Plasmid

    #60034

    Purpose

    Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-NCK (CRY2 Mustard Weed, Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_Xph15-GCaMP6f

    Plasmid

    #135538

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    GCaMP6f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. STK17A gRNA (BRDN0001149480)

    Plasmid

    #77916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK17A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK17A (STK17A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. STK17B gRNA (BRDN0001146764)

    Plasmid

    #76798

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK17B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK17B (STK17B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge