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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4221 - 4240 of 66674 results

  2. GFP-S100A11

    Plasmid
    #107201
    Purpose
    Expresses GFP-human S100A11 fusion protein for live cell imaging
    Depositor
    Volker Gerke, Ursula Rescher
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S100A11 (S100A11 Human)
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    107201-plasmid-map-sequence-id-209813

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  4. pJC30X-BS(X)

    Plasmid
    #58958
    Purpose
    Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Publication
    Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
    Tags
    First 8 codons of COX3
    Mutation
    GFP modified as described: CORMACK, B. P., R. H. …
    Promoter
    n/a
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. VN-SOD1

    Plasmid
    #89757
    Purpose
    expresses human SOD1 fused to the N-terminal part of Venus
    Depositor
    Tiago Outeiro
    Publication
    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SOD1 (SOD1 Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    89757-plasmid-map-sequence-id-178830

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  6. pαH-S-GSAS-B.1.351

    Plasmid
    #171749
    Purpose
    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Science. 2021 Jun 24. pii: science.abi62
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Available since
    July 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171749-plasmid-map-sequence-id-337764

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  7. p426TEF-LptTPS-A

    Plasmid
    #89468
    Purpose
    Expression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene production
    Depositor
    Jing-Ke Weng
    Insert
    LptTPS-A
    Tags
    none
    Expression
    Yeast
    Promoter
    pBR322 origin of replication for propagation in E…
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89468-plasmid-map-sequence-id-176087

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  8. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Publication
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  11. pαH-S-HRV3C

    Plasmid
    #164567
    Purpose
    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:
    Insert
    Spike - Furin site changed for HRV3C site
    Tags
    2X Strep-Tag II and 8X His tag
    Expression
    Mammalian
    Mutation
    682-685 (furin site) replaced with HRV3C site (LE…
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164567-plasmid-map-sequence-id-324831

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  13. pLenti Puro HA-Ubiquitin-WT

    Plasmid
    #236289
    Purpose
    Lentiviral vector expressing HA-tagged wild type ubiquitin
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236289-plasmid-map-sequence-id-467836

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  14. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid
    #157986
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157986-plasmid-map-sequence-id-332423

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Showing: 4221 - 4240 of 66674 results