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We narrowed to 12,189 results for: LEA

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Showing: 4221 - 4240 of 12189 results

  3. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Publication
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclearā€¦
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venuā€¦
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    222950-plasmid-map-sequence-id-450583

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  4. pBridge-tyrosinase.Ļƒ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 Ļƒ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 Ļƒ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nucā€¦
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substiā€¦
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  5. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Publication
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + Tā€¦
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    186626-plasmid-map-sequence-id-366906

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  6. pĪ±H-S-GSAS-B.1.617.2.v1

    Plasmid
    #182575
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N
    Depositor
    Priyamvada Acharya
    Publication
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage sā€¦
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRā€¦
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182575-plasmid-map-sequence-id-353646

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  7. pZE21/UBP1/ClpS

    Plasmid
    #98566
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon
    Depositor
    George Church
    Publication
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    In a synthetic operon downstream of UBP1 and Remoā€¦
    Promoter
    Same as UBP1 (operon) and Tet promoter (aTc inducā€¦
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98566-plasmid-map-sequence-id-203067

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  14. pICSL30022

    Plasmid
    #174586
    Purpose
    Level 0 N-terminal tag module carrying Twin Streptavidin uncleavable
    Depositor
    Mark Banfield
    Insert
    Twin Streptavidin uncleavable
    Use
    Synthetic Biology
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174586-plasmid-map-sequence-id-344732

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Showing: 4221 - 4240 of 12189 results