We narrowed to 30,980 results for: LEA

1. RFN_EGFP_82

   Plasmid

   #60074

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. RFN_EGFP_80

   Plasmid

   #60072

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pF(UG) hSyn NS34a-msGFP-'S-mito

   Plasmid

   #158768

   Purpose

   Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Flexible linkers between the protease and msGFP a…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCMV_HyPer7RgDAAO

   Plasmid

   #217653

   Purpose

   Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   HyPer7 D amino acid oxidase
   

   Use

   Lentiviral

   Tags

   Nuclear export signal

   Expression

   Mammalian

   Mutation

   Fused DAAO to the C-terminus of HyPer7 using a Gl…

   Promoter

   CMV

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa-LSS-GFP-LDLR wt

   Plasmid

   #98184

   Purpose

   Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

   Depositor

   Gary Banker, Marvin Bentley

   Article

   Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

   Insert

   LDLR (LDLR Human)
   

   Use

   Tags

   GFP and LDLR signal sequence, N-term of GFP so GF…

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta Actin

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pStreptagII 3C EGFR EABR

   Plasmid

   #234994

   Purpose

   For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   EGFR-EABR (EGFR Human)
   

   Use

   Tags

   Streptag-II, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHA StreptagII 3C NIS EABR

   Plasmid

   #234988

   Purpose

   For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NIS-EABR (SLC5A5 Human)
   

   Use

   Tags

   HA, Streptag-II, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

   Plasmid

   #208946

   Purpose

   A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

   Plasmid

   #208947

   Purpose

   A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mAzami-Green-Galectin

    Plasmid

    #102419

    Purpose

    Expresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.

    Depositor

    Geert van den Bogaart

    Article

    Dingjan et al Sci Rep. 2016 Feb 24;6:22064. doi: 10.10

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Tags

    mAzami-green

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. STT3A pEGFP-N2 (1051)

    Plasmid

    #62025

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid

    #97044

    Purpose

    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSc1-DD

    Plasmid

    #80439

    Purpose

    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    E. coli dihydrofolate reductase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

    Plasmid

    #208951

    Purpose

    A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

    Plasmid

    #208950

    Purpose

    A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

    Plasmid

    #208949

    Purpose

    A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

    Plasmid

    #208948

    Purpose

    A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNIC-ZB-SPRTN-E112A-FL

    Plasmid

    #110218

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PTRF/Cavin1_L (OZ513)

    Plasmid

    #27188

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pSc1

    Plasmid

    #80436

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge