Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

171,853 results

Showing: 10601 - 10650 of 171853 results

  1. HA-Clover-HIF-1alpha Wildtype

    Plasmid
    #163365
    Purpose
    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available Since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163365-plasmid-map-sequence-id-322246

    Enlarge

  2. pHS1950

    Plasmid
    #205983
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205983-plasmid-map-sequence-id-415374

    Enlarge

  5. PGKp-GFP-YAP (5SA)

    Plasmid
    #174174
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    PGK
    Available Since
    Aug. 19, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174174-plasmid-map-sequence-id-340277

    Enlarge

  8. pcDNA3.1-GGamma5-GFP2

    Plasmid
    #166777
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma5-GFP2 (GNG5 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166777-plasmid-map-sequence-id-408989

    Enlarge

  9. pcDNA3.1-GGamma3-GFP2

    Plasmid
    #166775
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma3-GFP2 (GNG3 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166775-plasmid-map-sequence-id-330996

    Enlarge

  11. RHCglo

    Plasmid
    #80169
    Purpose
    Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing
    Depositor
    Thomas Cooper
    Article
    Singh et al Biotechniques. 2006 Aug;41(2):177-81.
    Insert
    segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80169-plasmid-map-sequence-id-150808

    Enlarge

  13. SpCas9-ABE8e(V106W)

    Plasmid
    #198547
    Purpose
    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-ABE8e(V106W)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198547-plasmid-map-sequence-id-388882

    Enlarge

  15. TMED10-bio-His

    Plasmid
    #51852
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED10 (TMED10 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51852-plasmid-map-sequence-id-79739

    Enlarge

  24. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV1)

    Viral Prep
    #26966-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  29. B. subtilis Single Gene Deletion Library--Kanamycin

    Plasmid Kit
    #1000000115
    Purpose
    A collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
    Depositor
    Carol Gross
    Application
    Strain Collections
    Vector Type
    Bacterial Expression
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  32. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

    Enlarge

  37. pcDNA3.1-GGamma7-GFP2

    Plasmid
    #166778
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma7-GFP2 (GNG7 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available Since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166778-plasmid-map-sequence-id-331001

    Enlarge

  38. pYG026

    Plasmid
    #65328
    Purpose
    reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    ccdB
    Use
    Unspecified
    Available Since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    65328-plasmid-map-sequence-id-110561

    Enlarge

  39. TFORF0497

    Plasmid
    #143707
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EGR3 (EGR3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143707-plasmid-map-sequence-id-409085

    Enlarge

  43. rEstus

    Plasmid
    #209767
    Purpose
    To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.
    Depositor
    Stefan Heinemann
    Article
    Rühl et al bioRxiv 2023.
    Insert
    rEstus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209767-plasmid-map-sequence-id-413323

    Enlarge

  45. HA-HA-AR-DBD

    Plasmid
    #171238
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171238-plasmid-map-sequence-id-336038

    Enlarge

  47. pAAV-Ef1a-fDIO EYFP (AAV Retrograde)

    Viral Prep
    #55641-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  49. pRRlsinPGK_H2BGFP_WPRE

    Plasmid
    #91788
    Purpose
    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
    Depositor
    Beverly Torok-Storb
    Insert
    Human histone H2B (H2BC11 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    91788-plasmid-map-sequence-id-180614

    Enlarge

Showing: 10601 - 10650 of 171853 results