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Silene_conica _GlnRS_MitoTP_pK7FWG2

Plasmid

#202654

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _LysRS_MitoTP_pK7FWG2

Plasmid

#202655

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Agrostemma_githago _LysRS_MitoTP_pK7FWG2

Plasmid

#202656

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _TyrRS_MitoTP_pK7FWG2

Plasmid

#202657

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_vulgaris _TyrRS_MitoTP_pK7FWG2

Plasmid

#202658

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Silene_conica _PheRS_MitoTP_pK7FWG2

Plasmid

#202659

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Silene_conica _PheRS_PlastidTP_pK7FWG2

Plasmid

#202660

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

Plasmid

#202661

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

Insert

N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD-P19-EGFP

Plasmid

#196609

Purpose

siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + EGFP inverted repeat sequence

Tags

6x His tag

Expression

Bacterial

Promoter

AraC

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-P19-NP

Plasmid

#196608

Purpose

siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

p19 + Influenza virus NP inverted repeat

Tags

6x His tag

Expression

Bacterial

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-Fzd1-IRES-tdTomato (TET006)

Plasmid

#160510

Purpose

This plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al bioRxiv 2022

Insert

Fzd1 (Fzd1 Mouse)

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP8EFA

Plasmid

#185854

Purpose

Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

DHFR P66L

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ZmIAA29 (yeast codon optimized)

Plasmid

#188441

Purpose

Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Repressor IAA29 (yeast codon optimized)

Use

Cloning

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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1056H

Plasmid

#183135

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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1056J

Plasmid

#183136

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1056K

Plasmid

#183137

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJCC_103 SpCas9 T1337C

Plasmid

#179525

Purpose

For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

Insert

SpCas9 T1337C

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed threonine 1337 to cysteine

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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