We narrowed to 48,864 results for: des.1

1. pIRES2-eGFP:HA-mTREK-1(tandem wt-S131C)

   Plasmid

   #224778

   Purpose

   Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 wt-S131C) (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S562C

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRES2-eGFP:HA-mTREK-1(tandem wt-wt)

   Plasmid

   #224776

   Purpose

   Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3- SREBP-1 (A317-318;A341; A1171)

   Plasmid

   #204196

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317,318,341,1171

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCA528 KATNA1 MIT residues 1-79, V55D

   Plasmid

   #180615

   Purpose

   Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-79, V55D mutation

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB80-hsKIF5B(1-353)GCN4-L-GFP

   Plasmid

   #193714

   Purpose

   Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353);

   Promoter

   Chicken beta-actin

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

   Plasmid

   #193715

   Purpose

   Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Synthetic, Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-353); mCherry: Met1 Deleted

   Promoter

   Chicken beta-actin

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GFP-IRES-mSNCA(1-95)

   Plasmid

   #185716

   Purpose

   AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   deleted AA 95-144

   Promoter

   hSyn

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

   Plasmid

   #174635

   Purpose

   Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
   

   Use

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB80-KIF1A(1-383)-GFP-SSPB(micro)

    Plasmid

    #174627

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Use

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1

    Plasmid

    #112841

    Purpose

    transient/stable overexpression of the N-terminal domain of Catulin.

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Catulin-alpha-1 (Ctnnal1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N domain of Catulin

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS3-MT-BX-6MYC p21 1-89

    Plasmid

    #78784

    Purpose

    To overexpress p21 1-89 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    6xMYC

    Expression

    Mammalian

    Mutation

    Jan-89

    Promoter

    CMV IE94

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

13. HLH Bmal Pas 1-465-VenC L95E

    Plasmid

    #47365

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, L95E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

14. HLH Bmal Pas 1-465-VenC L150E

    Plasmid

    #47366

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, L150E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. HLH Bmal Pas 1-465-VenC F423R

    Plasmid

    #47368

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, F423R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

16. HLH Bmal Pas 1-465-VenC W427A

    Plasmid

    #47369

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, W427A

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

17. HLH Bmal Pas 1-465-VenC V435R

    Plasmid

    #47370

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, V435R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid

    #188072

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Use

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560, deletion of aa2-6

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGC-1 alpha promoter luciferase delta CRE

    Plasmid

    #8888

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Site-directed mutagenesis to remove the CREB bind…

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGC-1 alpha promoter luciferase delta MEF

    Plasmid

    #8889

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter dMEF (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Site-directed mutagenesis to remove the MEF2 bind…

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge