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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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2xP53_RE::NLuc

Plasmid

#124533

Purpose

p53 NLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

P53 NLuc reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUBQ:Citrine-TurboID-3xMyc

Plasmid

#199244

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID-3xMyc

Tags

TurboID-3xMyc

Expression

Plant

Promoter

Arabidopsis UBQ10 promoter with intron

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCF1148

Plasmid

#222330

Purpose

Fluorescent reporter plasmid for Veillonella

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Insert

bs2 gene with Veillonella parvula SKV38 mdh promoter

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Promoter

Veillonella parvula SKV38 mdh promoter

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-MKLP1

Plasmid

#70145

Purpose

mammalian expression of MKLP1 with an eGFP fusion

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE FLAG-IKK2-EE puro

Plasmid

#216790

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

IKK-2 (IKBKB Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Promoter

LTR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-AT

Plasmid

#133306

Purpose

"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

axe/txe toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-dCas9

Plasmid

#47106

Purpose

Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Flag and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive)

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

Plasmid

#101209

Purpose

Bacterial expression plasmid for SpCas9-HF1 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A, Q695A and Q926A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-NG

Plasmid

#138159

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pB-MultiplexedReporter-MOR170-1

Plasmid

#129461

Purpose

piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

MOR170-1 (Olfr895 Mouse)

Use

Synthetic Biology

Tags

LUCY tag, Rho tag

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pV1393

Plasmid

#111430

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TNPO1-shRNA

Plasmid

#86082

Purpose

A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-RIG-I-Myc

Plasmid

#215872

Purpose

RIG-I-Myc expression vector for overexpression

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

Insert

RIG-I (RIGI Human)

Expression

Mammalian

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3alpha2_35S:P19:Tnos (GB1203)

Plasmid

#68214

Purpose

Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

P19 silencing suppressor

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGPTVII-Bar-U-MatryoshCaMP6s

Plasmid

#100024

Purpose

Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Expression

Plant

Promoter

AtUBQ10

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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7xE-Box::Renilla

Plasmid

#124532

Purpose

c-Myc Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

c-Myc Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_mic

Plasmid

#184920

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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