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Plasmid#90240PurposeLentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (AE mutant)
UseLentiviralTagsmCloverExpressionMammalianPromoterPGKAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 mCit mCer
Plasmid#83563PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPRKCA (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE257 pCDNA3.1(zeo)-hDsh3-truncDEP
Plasmid#16752DepositorInsertFLAG-hDsh3-trunc before DEP (DVL3 Human)
TagsflagExpressionMammalianMutationtruncated before DEPAvailable SinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ctrl)
Plasmid#69215PurposeAdvanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNADepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV promoter
P2A-mNeonGreen
UseCRISPR and LentiviralTagsFLAGAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant PKA-Cα-mEGFP
Plasmid#114143PurposeMammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
ExpressionMammalianAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Caveolin3DGV
Plasmid#67886PurposeFluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cellsDepositorInsertCav3 (Cav3 Mouse)
TagsEGFPExpressionMammalianMutationDeletion of the first 53 amino acids of Caveolin3PromoterCMVAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
DF-UAS-sspa-GCaMP6f
Plasmid#67569PurposeZebrafish expression of a short photoactivatable fluorescent calcium reporterDepositorInsertsspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…MutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
shYES1 # 1
Plasmid#42546DepositorAvailable SinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
bu6-sgCebpa_v2-mU6-sgCebpb_v2-hU6-sgCebpd_v2
Plasmid#177258PurposeExpresses Cebpa_v2 (bU6), Cebpb_v2 (mU6), Cebpd_v2 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgCebpa_v2/sgCebpb_v2/sgCebpd_v2
UseLentiviralPromoterbU6/mU6/hU6Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-REX-GECO0.9
Plasmid#61247PurposeExpresses REX-GECO0.9 in mammalian cellsDepositorInsertREX-GECO0.9
ExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterCMVAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ
Plasmid#16754DepositorInsertFLAG-hDsh3-trunc before PDZ (DVL3 Human)
TagsflagExpressionMammalianMutationtruncated before PDZAvailable SinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPN098
Plasmid#91625PurposeExpress sgRNA targeting human HCN1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN099
Plasmid#91626PurposeExpress sgRNA targeting human HCN1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
DF-UAS-spa-GCaMP6f
Plasmid#67568PurposeZebrafish expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…TagsHIS-tagMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…Available SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPN234
Plasmid#91591PurposeExpress sgRNA targeting human CYFIP1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN262
Plasmid#91607PurposeExpress sgRNA targeting human EPHX2DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
49535-sgFMR1_E17A
Plasmid#157782PurposeExpresses a sgRNA targeting stop codon of human FMR1 geneDepositorAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPN107
Plasmid#91636PurposeExpress sgRNA targeting human KDM4ADepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only