We narrowed to 5,259 results for: PID

1. pVITRO1-Trastuzumab-IgG3/κ

   Plasmid

   #61885

   Purpose

   Expresses HER2/neu receptor specific humanized IgG3/κ antibody isotype (Trastuzumab-IgG3/κ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   HER2/neu receptor specific humanized Gamma 3 heavy chain expression cassette
   HER2/neu receptor specific humanized kappa light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mEF1 Prom and rEF1 Prom

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-EFIRES-P-GFP(1-10)opti

   Plasmid

   #129416

   Purpose

   Expressing codon-optimized GFP(1-10) fragment in human cells

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   codon-optimized GFP(1-10)
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-CRY2-CreN

   Plasmid

   #26888

   Purpose

   Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2-CreN (cre, CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 19-104 of Cre

   Promoter

   CMV

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP434

   Plasmid

   #104167

   Purpose

   Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
   

   Use

   Tags

   14x Histidine tag and NEDD8 from Brachypodium dis…

   Expression

   Bacterial

   Mutation

   3x Cysteines located at bps 493-495, 520-522, and…

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-2xPH-TAPP1 R211L mutant

   Plasmid

   #161991

   Purpose

   Expresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   PH-TAPP1 (PLEKHA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R211L

   Promoter

   Available since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVITRO1-102.1F10-IgG4/λ

   Plasmid

   #50369

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR221-IL6.Pro

   Plasmid

   #79491

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Il6 Promoter (IL6 Synthetic, Human)
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   IL6 Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVITRO1-102.1F10-IgA2/λ

   Plasmid

   #50371

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO1-dV-IgE/κ

    Plasmid

    #61878

    Purpose

    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23 A82V 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86016

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Pardis Sabeti, Jeremy Luban

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 82 to valine

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid

    #107581

    Purpose

    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVITRO1-102.1F10-IgG2/λ

    Plasmid

    #50367

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVITRO1-102.1F10-IgG3/λ

    Plasmid

    #50368

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVITRO1-102.1F10-IgA1/λ

    Plasmid

    #50370

    Purpose

    Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMDC43-FIT2

    Plasmid

    #96993

    Purpose

    Express GFP-tagged mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    E2D (please see depositor comments below)

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. JWW-2 human chimeric monoclonal antibody

    Plasmid

    #66749

    Purpose

    Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-2 Heavy Chain
    JWW-2 Light Chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 and rEF1

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLHCX-mPM20D1-flag

    Plasmid

    #84566

    Purpose

    mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

    Depositor

    Bruce Spiegelman

    Article

    Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

    Insert

    peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    Retroviral

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES2-EGFP-SHP2DA

    Plasmid

    #12286

    Depositor

    Anton Bennett

    Article

    Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

    Insert

    SHP-2DA (PTPN11 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Aspartic Acid 425 mutated to Alanine

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-mitf

    Plasmid

    #69801

    Purpose

    Used to make mitf knockout porcine cell line

    Depositor

    Jianguo Zhao

    Article

    Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

    Inserts

    mitf-sgRNA
    SpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-Actin and U6

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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