We narrowed to 5,339 results for: pcdna3.1

1. HiBiT-FZD5

   Plasmid

   #195843

   Purpose

   Expresses human FZD5 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD5 (FZD5 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HiBiT-FZD3

   Plasmid

   #195841

   Purpose

   Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD3 (FZD3 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   I292V and V495I

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-D2s-L-V1

   Plasmid

   #19967

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-N terminal Venus (DRD2 Aequorea Victoria, Human)
   

   Tags

   Flag and N terminal Venus

   Expression

   Mammalian

   Available Since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

4. FLAG HDAC5 S259A/S498A

   Plasmid

   #32216

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser259Ala, Ser498Ala

   Promoter

   CMV

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

5. CoV2-N-G204R

   Plasmid

   #177953

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (G204R) (N )
   

   Expression

   Mammalian

   Mutation

   G204R

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLYLQ

   Plasmid

   #80421

   Purpose

   expresses cDNA for human CELF1 (CUGBP1) Flag tagged

   Depositor

   Thomas Cooper

   Article

   Kalsotra et al Proc Natl Acad Sci U S A. 2008 Dec 23;10

   Insert

   human CELF1 cDNA (CELF1 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-LYRa11-RBD-8h

   Plasmid

   #161822

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T324-D522)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATG9A-2STrep

   Plasmid

   #210359

   Purpose

   Expresses ATG9A fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   ATG9A (ATG9A Human)
   

   Tags

   2Strep

   Expression

   Mammalian

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB07 (CK2beta)

   Plasmid

   #27091

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CaMKIV-DCTK75E

    Plasmid

    #126423

    Purpose

    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed lysine 75 to glutamic acid

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNATM3.1/V5-His TOPO-PKCi-K274W

    Plasmid

    #55690

    Purpose

    expresses dominant negative human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E and K274W

    Promoter

    CMV

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Nuc-mTurq2-Apollo-NADP+

    Plasmid

    #193302

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    SV40 NLS and mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-GAS41

    Plasmid

    #15428

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    GAS41 (Yeats4 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA 3.1-nsp7-FLAG

    Plasmid

    #201027

    Purpose

    Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    8xHis and FLAG

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid

    #55687

    Purpose

    expresses constitutively active human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E

    Promoter

    CMV

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-Rs7327-RBD-8h

    Plasmid

    #161823

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Opto-GPR4

    Plasmid

    #106001

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR4 (GPR4 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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