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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 4321 - 4340 of 14582 results

  1. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139991-plasmid-map-sequence-id-275523

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  2. pGL3-Basic-hCYP26A1-FL- luciferase

    Plasmid
    #135566
    Purpose
    Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase
    Depositor
    Catharine Ross
    Publication
    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Full-length promoter of human retinoic acid receptor-beta gene
    Promoter
    hHuman CYP26A1 gene promoter
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135566-plasmid-map-sequence-id-268053

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  8. AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

    Plasmid
    #205445
    Purpose
    human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT
    Depositor
    Deepta Bhattacharya
    Publication
    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iCasp9-Crry-mCD59-Qa1 SCT
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205445-plasmid-map-sequence-id-408940

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  9. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Publication
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  10. pcDNA3-sACE2v6-sfGFP

    Plasmid
    #145177
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145177-plasmid-map-sequence-id-292606

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Showing: 4321 - 4340 of 14582 results