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LentiCRISPR v2-sgAMPK alpha1 clone3

Plasmid

#162121

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha1

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha1 (PRKAA1 Human)

Use

Lentiviral

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf78

Plasmid

#12766

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf78

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Socs5-SH2

Plasmid

#46508

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs5 (SOCS5 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 378-481

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Grap-SH2

Plasmid

#46434

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap (GRAP Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 51-160

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLPC-N-Myc-Flag-BirA-hPOT1

Plasmid

#166409

Purpose

express BirA-hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Tags

BirA, flag, and myc

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag_cx

Plasmid

#167273

Purpose

Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Mutation

P179R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

Plasmid

#166412

Purpose

express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 K90E (POT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

K90E

Promoter

EF-1a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-EBFP2-MiniWhite

Plasmid

#165918

Purpose

Blasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf35

Plasmid

#12723

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf35 (J3R Vaccinia Virus)

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Cten-SH2

Plasmid

#46421

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cten (TNS4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 444-570

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf86

Plasmid

#12774

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf86

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Stat2-SH2

Plasmid

#46512

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat2 (STAT2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 566-681

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pMSP1D1T2

Plasmid

#97328

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1T2

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of two T residues after T25

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX ShcC-SH2

Plasmid

#46487

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcC (SHC3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 485-594

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf144

Plasmid

#12832

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf144

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
Psicheck2 Sox4 mut 3'UTR oligo

Plasmid

#26988

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

Sox4 mut UTR oligo (SOX4 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

bp 449-509 of SOX4 3'UTR; mut @ bp 483

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-SMO-1(GG)

Plasmid

#87054

Purpose

Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

smo-1 (smo-1 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The last aa was removed to render processed SMO-1…

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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