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pAAV-SynI-FlpOn-CreOff-TdTomato

Plasmid

#176275

Purpose

Viral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Publication

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

tdTomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

Plasmid

#127864

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Rat SARE-Arc Min Promoter-D2GFP-SV40 3'UTR

Plasmid

#233058

Purpose

To express D2GFP from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native SV40 3'UTR containing two introns

Depositor

Jonathan Ploski

Insert

D2GFP (Arc Rat, Synthetic)

Use

AAV

Available since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Nsp2Cas9_AAV

Plasmid

#192143

Purpose

Expresses Nsp2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Publication

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nsp2Cas9

Use

AAV

Expression

Mammalian

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKII:RiboL1-jGCaMP8m

Plasmid

#239668

Purpose

Expresses soma-targeted (ribosome tethered via RiboL1) jGCaMP8m under CaMKII promoter.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has service

AAV8

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

CaMKII

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKII:RiboL1-jGCaMP8s

Plasmid

#239667

Purpose

Expresses soma-targeted (ribosome tethered via RiboL1) jGCaMP8s under CaMKII promoter.

Depositor

Marianne Fyhn

Publication

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

CaMKII

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-HGM (optimized)

Plasmid

#220988

Purpose

Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Publication

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

HaloTag-GFP-mito

Use

AAV

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jGCaMP7b-XC

Plasmid

#182221

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Publication

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

AAV

Expression

Mammalian

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-KrasWT/sgKras/Cre

Plasmid

#99848

Purpose

Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Publication

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244092

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244099

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Publication

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1s-ST-WPRE

Plasmid

#214968

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

Depositor

Peng Zou

Publication

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1s-ST

Use

AAV

Promoter

human Synapsin

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_r5-HTmut

Plasmid

#208720

Purpose

Expresses the red 5-HT sensor GRAB_r5-HTmut in neurons

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HTmut

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Publication

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

EFS

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSyn1-EGFP

Plasmid

#153187

Purpose

Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Publication

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI3sub-Kv-WPRE

Plasmid

#246616

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub-Kv

Use

AAV and Cre/Lox

Tags

Kv

Expression

Mammalian

Promoter

EF1a

Available since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

Plasmid

#184635

Purpose

Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Publication

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV pSYN eGFP T2A SP HALO HAPLN1

Plasmid

#228199

Purpose

AAV vector expressing GFP and HaloTag-HAPLN1 under synaptophysin promoter

Depositor

Sungjin Park

Publication

Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

Insert

HAPLN1 (Hapln1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human synaptophysin promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Dlx-FLEX-hM4Di-mCherry

Plasmid

#209701

Purpose

Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

Depositor

Peer Wulff

Publication

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

hM4D(Gi)

Use

AAV

Tags

mCherry

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Publication

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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