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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,355 results for: construct

Showing: 4341 - 4360 of 8355 results

  1. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PR promoter and PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

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  2. KTK_139

    Plasmid
    #180565
    Purpose
    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    180565-plasmid-map-sequence-id-373326

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  3. KTK_113

    Plasmid
    #180540
    Purpose
    Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    Signal peptide region of CsgA including SecB-N22 sequenced
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180540-plasmid-map-sequence-id-369685

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  4. KTK_095

    Plasmid
    #180524
    Purpose
    dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180524-plasmid-map-sequence-id-359542

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  5. KTK_324

    Plasmid
    #180566
    Purpose
    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180566-plasmid-map-sequence-id-358835

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  6. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid
    #153439
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153439-plasmid-map-sequence-id-306439

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  7. MTK0_004

    Plasmid
    #123961
    Purpose
    Encodes the Cas9 cROSA26 homology destination with Blasticidin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    cRosa26 destination
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    123961-plasmid-map-sequence-id-272155

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  8. ARB287d

    Plasmid
    #124018
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-spacer_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124018-plasmid-map-sequence-id-252457

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  9. ARB287e

    Plasmid
    #124019
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-spacermulticistronic_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124019-plasmid-map-sequence-id-252460

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  10. JPF0464

    Plasmid
    #124035
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_non targeting sgRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124035-plasmid-map-sequence-id-252506

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  11. JPF0465

    Plasmid
    #124036
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_mScarlet sgRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124036-plasmid-map-sequence-id-252507

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  12. JPF0466

    Plasmid
    #124037
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_tagBFP sgRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124037-plasmid-map-sequence-id-262668

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  13. JPF0468

    Plasmid
    #124038
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124038-plasmid-map-sequence-id-252546

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  17. ARB287a

    Plasmid
    #124015
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124015-plasmid-map-sequence-id-252435

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  18. ARB287b

    Plasmid
    #124016
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-SV40pA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124016-plasmid-map-sequence-id-252439

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  19. ARB287c

    Plasmid
    #124017
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-rglpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124017-plasmid-map-sequence-id-252443

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Showing: 4341 - 4360 of 8355 results