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GFP-KIF1B beta-600-1400

Plasmid

#79109

Purpose

Mammalian expression of GFP-KIF1B beta-600-1400

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter mir30-5

Plasmid

#46692

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir30-5 (MIR30A Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-10-3xFLAG-LGP2-MIII

Plasmid

#58683

Purpose

Expresses LGP2 with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

LGP2-MIII (DHX58 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

threonine and serine of helicase motif III mutate…

Promoter

CMV

Available Since

July 31, 2014

Availability

Academic Institutions and Nonprofits only
pCS2+ Flag

Plasmid

#237457

Purpose

Vector for constitutive mammalian expression of gene of interest

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Type

Empty backbone

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Promoter

PhmtB

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGL1_37

Plasmid

#199044

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL1_39

Plasmid

#199046

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI plus NGS barcode 16

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG mCherry-LDTS-LIRmut

Plasmid

#189003

Purpose

lipophagy-inducing adaptor protein, AAV, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut, mCherry

Use

AAV

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep-GFP11

Plasmid

#153514

Purpose

TwinStrep-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8383

Plasmid

#126917

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_hSyn_Ilmol

Plasmid

#120311

Purpose

Expresssion of Ilmol preferentially in neurons

Depositor

Bradley Baker

Article

Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

Insert

Ilmol

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-PseB-His6

Plasmid

#89723

Purpose

Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseB

Tags

His6

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(WT)

Plasmid

#111323

Purpose

for T7 transcription of wt actin pre-mRNA

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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POT5-CYC1p-ycrtYB-ADH1t

Plasmid

#65360

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtYB-ADH1t

Use

Unspecified

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
BLA_CAT_K136_GPG_LOV2

Plasmid

#231575

Purpose

This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Insert

CAT_ carrying an AsLOV2 insertion behind K136

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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MbCas12a_N1153_GPG_GR2

Plasmid

#231578

Purpose

Encodes a cortisol inducible variant of MbCas12a

Depositor

Dominik Niopek

Insert

MbCas12a with a GR2 insertion behind N1153

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Expression

Bacterial

Mutation

R632S

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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