We narrowed to 4,455 results for: TIL

1. pHalo-MyosinIIA-C25

   Plasmid

   #128573

   Purpose

   Studies of cellular contractility

   Depositor

   Dylan Burnette

   Article

   Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

   Insert

   Myosin IIA (Myh9 Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

   Plasmid

   #60418

   Purpose

   Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   mTiam1-tgRFPt-SSPB R73Q
   

   Use

   Cre/Lox and Lentiviral

   Tags

   tgRFPt

   Expression

   Mammalian

   Mutation

   mTiam 64-437 // SSPB R73Q, Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCXIP GFP-MOSPD2 RD/LD

   Plasmid

   #186468

   Purpose

   Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R404D/L406D (mutant unable to bind FFAT motifs)

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF3537

   Plasmid

   #145013

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TSC22D4 (TSC22D4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p20StAD

   Plasmid

   #190268

   Purpose

   Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

   Depositor

   Assaf Mosquna

   Article

   Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast genome-integrating vector

   Tags

   GAL4-AD

   Expression

   Mutation

   Promoter

   TDH3

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOCK CM-R03

   Plasmid

   #46567

   Purpose

   Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).

   Depositor

   Clifford Wang

   Article

   Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

   Insert

   CMV mutant promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Mutant version of CMV promoter (refer to Table 1 …

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCOCK CMVwt

   Plasmid

   #46559

   Purpose

   Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

   Insert

   CMV wt promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Our version has a G at base 724 (reference has C)

   Promoter

   CMV

   Available since

   Sept. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCOCK CM-R05

   Plasmid

   #46568

   Purpose

   Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).

   Depositor

   Clifford Wang

   Article

   Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

   Insert

   CMV mutant promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Mutant version of CMV promoter (refer to Table 1 …

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCOCK CM-C02

   Plasmid

   #46563

   Purpose

   Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).

   Depositor

   Clifford Wang

   Article

   Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

   Insert

   CMV mutant promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Mutant version of CMV promoter (refer to Table 1 …

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCOCK CM-C18

    Plasmid

    #46560

    Purpose

    Mammalian expression plasmid with EGFP expressed from synthetic promoter (CMVwt CAAT box mutant).

    Depositor

    Clifford Wang

    Article

    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

    Insert

    CMV mutant promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Mutant version of CMV promoter (refer to Table 1 …

    Promoter

    Available since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

    Plasmid

    #208872

    Purpose

    For expression in E. coli of NAP1 (delta-NDP52)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    S37K/A44E (disrupts NDP52 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

    Plasmid

    #208873

    Purpose

    For expression in E. coli of NAP1 (delta-TBK1)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

    Plasmid

    #208863

    Purpose

    Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    S37K/A44E (disrupts NDP52 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

    Plasmid

    #208864

    Purpose

    Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    I11S/L12S (disrupts FIP200 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

    Plasmid

    #208865

    Purpose

    Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCRISPR-cBEST-v2-SF14P

    Plasmid

    #209448

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from SF14P promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR-cBEST-v2-kasOP*

    Plasmid

    #209447

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from kasOP* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p21StBD

    Plasmid

    #190269

    Purpose

    Constitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.

    Depositor

    Assaf Mosquna

    Article

    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast genome-integrating vector

    Tags

    GAL4-BD

    Expression

    Mutation

    Promoter

    TDH3

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b MOSPD2 [282-490]

    Plasmid

    #186469

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Human_TFAM_HMGB_ctail_pET28

    Plasmid

    #167330

    Purpose

    Expresses TFAM HMGB+C-tatil mutant in bacterial cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Truncation mutant containing HMGB and C-tail doma…

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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