Addgene

We narrowed to 724 results for: CRN;

Showing: 631 - 660 of 724 results

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mc2 mCherry hPGK

Plasmid

#206231

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 blast hPGK

Plasmid

#206229

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro hPGK

Plasmid

#206228

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 puro hPGK

Plasmid

#206227

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 neo hPGK

Plasmid

#206226

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 hygro EF1a

Plasmid

#206222

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 hygro

Plasmid

#206216

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT353

Plasmid

#204506

Purpose

In vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

CeRep55 tandem repeats from Y73B3A (C. elegans)

Use

Other

Tags

Expression

Mutation

Promoter

double-T7

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSLS.539

Plasmid

#184958

Purpose

Test effect of a1/a2 length on editing fabH using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

fabH donor G954A

Promoter

T7/lac

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.031

Plasmid

#184968

Purpose

Test effect of extending -Eco2 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(extended), a1/a2 length: 29

Use

Tags

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.544

Plasmid

#184961

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22

Use

Tags

Expression

Bacterial

Mutation

murF donor G1359A, a1/a2 length extended to 22 bp

Promoter

T7/lac

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.543

Plasmid

#184960

Purpose

Test effect of a1/a2 length on editing murF using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

murF donor G1359A

Promoter

T7/lac

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.545

Plasmid

#184962

Purpose

Test effect of a1/a2 length on editing priB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

priB donor G1069A

Promoter

T7/lac

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8

Plasmid

#176184

Purpose

gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8_28bp

Plasmid

#176185

Purpose

plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL9

Plasmid

#176186

Purpose

2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_MCP-VP64_D-MS2

Plasmid

#177161

Purpose

Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

MCP-VP64
D-MS2

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_PCP-VP64_A-PP7

Plasmid

#177156

Purpose

Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

PCP-VP64
A-PP7

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_MCP-VP64_C-MS2

Plasmid

#177160

Purpose

Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

MCP-VP64
C-MS2

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1H07

Plasmid

#71031

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

exd (exd Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
S1m tagged DANCR

Plasmid

#138585

Purpose

For in vivo tagging

Depositor

Yin-Yuan Mo

Article

Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

Insert

DANCR (DANCR Human)

Use

Lentiviral

Tags

S1m tag

Expression

Mutation

Promoter

MSCV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C-GFP

Plasmid

#195154

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3-B-pGAS5

Plasmid

#46371

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

GAS5 (GAS5 Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

promoter

Promoter

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPERIORpuro-shGAS5

Plasmid

#46370

Depositor

Rolf Stahel, Emanuela Felley-Bosco

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

GAS5 (GAS5 Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

H1

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pAG193 CORO1C 3'UTR

Plasmid

#11998

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

CORO1C 3'UTR (CORO1C Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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D-raf R217L

Plasmid

#39762

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Use

Tags

Expression

Insect

Mutation

R217L

Promoter

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pAG143 AK024929 3'UTR

Plasmid

#12001

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N8 3'UTR (LINC00341 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C DCC-GFP

Plasmid

#195149

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove coi…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C DCC -Halo

Plasmid

#195150

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove coi…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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