Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,317 results for: LEA

Clear keywords
Showing: 421 - 440 of 12317 results

  5. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

    Plasmid
    #123353
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean3-Cerulean3-FLARE-Cameleon
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123353-plasmid-map-sequence-id-307189

    Enlarge

  7. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114274-plasmid-map-sequence-id-218322

    Enlarge

  17. M-ST1n-VP64

    Plasmid
    #48675
    Purpose
    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null (D9A D598A H599A N622A)
    Promoter
    CMV
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48675-plasmid-map-sequence-id-71850

    Enlarge

  18. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58477-plasmid-map-sequence-id-90386

    Enlarge

  19. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)

    Viral prep
    #51084-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jonathan Ting
    Promoter
    Syn
    Tags
    nuclear dTomato (physically separate, not a fusion protein)
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 421 - 440 of 12317 results