We narrowed to 1,481 results for: NEB

1. pminiT-Halo-MED1 donor

   Plasmid

   #197868

   Purpose

   Donor plasmid used for building Halo-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Halotag

   Expression

   Mutation

   Promoter

   NA

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACS_19

   Plasmid

   #111651

   Purpose

   Funclib design ACS_19

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   ACS_19
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTXB1-xlH2B(1-114)_K114A

   Plasmid

   #118217

   Purpose

   Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   xlH2B(1-114)
   

   Use

   Tags

   Mxe-GyrA intein fused to chitin binding domain

   Expression

   Bacterial

   Mutation

   Lysine 114 to alanine

   Promoter

   T7

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pminiT-mEmerald-MED1 donor

   Plasmid

   #197867

   Purpose

   Donor plasmid used for building mEmerald-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   NA

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MT2a_K30A K31A

   Plasmid

   #105696

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant

   Depositor

   Artur Krężel

   Article

   Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

   Insert

   Metallothionein 2a (MT2A Human)
   

   Use

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Mutation

   Lysine 30 and 31 to Alanine

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTE_25

   Plasmid

   #111638

   Purpose

   Funclib design PTE_25

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_25
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PTE_26

   Plasmid

   #111639

   Purpose

   Funclib design PTE_26

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_26
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PTE_27

   Plasmid

   #111640

   Purpose

   Funclib design PTE_27

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_27
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PTE_28

   Plasmid

   #111641

   Purpose

   Funclib design PTE_28

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   PTE_28
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PTE_36

    Plasmid

    #111642

    Purpose

    Funclib design PTE_36

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_36
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PTE_27.14

    Plasmid

    #111643

    Purpose

    Funclib design PTE_27.14

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_27.14
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ACS_4

    Plasmid

    #111646

    Purpose

    Funclib design ACS_4

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    ACS_4
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    see depositor comments below

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ACS_9

    Plasmid

    #111647

    Purpose

    Funclib design ACS_9

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    ACS_9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dPTE2

    Plasmid

    #111634

    Purpose

    PROSS stabilized PTE S5

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    dPTE2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PTE_5

    Plasmid

    #111635

    Purpose

    Funclib design PTE_5

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_5
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PTE_13

    Plasmid

    #111636

    Purpose

    Funclib design PTE_13

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_13
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PTE_24

    Plasmid

    #111637

    Purpose

    Funclib design PTE_24

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_24
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (SUMO)5-(SIM)10

    Plasmid

    #126923

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    internal BamHI site between (SUMO)5 and (SIM)10

    Promoter

    tac

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid

    #89154

    Purpose

    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge