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RCH1:GA2ox2-GFP

Plasmid

#108567

Purpose

Arabidopsis transformation, expresses GA2ox2 with RCH1 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

GA2ox2 (GA2OX2 Mustard Weed)

Expression

Plant

Promoter

RCH1

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHDT1:GUS

Plasmid

#108441

Purpose

Arabidopsis transformation, expresses HDT1 promoter/GUS fusion to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT1 promoter (HDA3 Mustard Weed)

Tags

GUS

Expression

Plant

Promoter

HDT1

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJM103: 150bp Hps2 RFP on pCM66T

Plasmid

#74744

Purpose

pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)

Depositor

Mary Lidstrom

Insert

RFP (BioBrick part number BBa_E1010)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pHps2, trimmed (extracted from Methylovorus gluco…

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pIS53 SERP1 3'UTR mut

Plasmid

#14500

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR mutant (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
PB-LYST-AlfaTag

Plasmid

#253252

Purpose

Overexpress LYST with a C-terminal AlfaTag.

Depositor

May Malicdan

Article

Serra-Vinardell et al Cell Mol Life Sci. 2023 Jan 28;80(2):53.

Insert

LYST (LYST Human)

Use

Piggybac transposon

Tags

AlfaTag

Expression

Mammalian

Promoter

CAG

Available Since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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Suntag_dPRDM9_FWA_g4

Plasmid

#249562

Purpose

dCas9_dPRDM9 catalytically dead version

Depositor

Jake Harris

Article

Binenbaum et al Nat Commun. 2025 Oct 31;16(1):9587. doi:

Insert

PRDM9 (Prdm9 Mouse)

Use

Synthetic Biology

Tags

HA and sfGFP

Expression

Plant

Mutation

G282A (Numbering from the the full length sequenc…

Promoter

UBQ10

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.neo GFP shDNM1L

Plasmid

#220356

Purpose

Retroviral expression vector for an shDNM1L

Depositor

David Kashatus

Article

Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

Insert

shDNM1L (DNM1L Human)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHDT1:HDT1-GFP

Plasmid

#108565

Purpose

Arabidopsis transformation, expresses HDT1/GFP fusion with HDT1 promoter to study expression pattern in roots

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Insert

HDT1 (HDA3 Mustard Weed)

Tags

GFP

Expression

Plant

Promoter

HDT1

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTH729-minRLuc

Plasmid

#38225

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Mutation

Every codon of the Renilla coding sequence was ex…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pIS57 PGM2 3'UTR mut

Plasmid

#14492

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

PGM2 3'UTR mutant (PGM2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS56 NETO2 3'UTR mut

Plasmid

#14496

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

NETO2 3'UTR mutant (NETO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS58 TIP120A 3'UTR mut

Plasmid

#14502

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

TIP120A 3'UTR mutant (CAND1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA4

Plasmid

#164934

Purpose

Expresses EGFP sgRNA4 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-I248V

Plasmid

#201554

Purpose

Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Expression

Bacterial

Mutation

changed Isoleucine 248 to Valine (numbering based…

Promoter

RBS

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

Plasmid

#201552

Purpose

Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1

Tags

HaloTag

Expression

Insect

Mutation

changed lysine 282 to arginine (numbering based o…

Promoter

Ac5

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIS55 POGK 3'UTR mut

Plasmid

#14498

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

POGK 3'UTR mutant (POGK Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

First, fourth, and fifth occurrences of CATTCC’s …

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

Plasmid

#231233

Purpose

MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Mutation

TAD deleted (delta1-143); numbering based on sequ…

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138E

Plasmid

#208287

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138E

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Glu

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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OptoProfilin.S138A

Plasmid

#208288

Purpose

Expresses Cry2(1-531)-mCherry-Profilin.S138A

Depositor

Robert Hughes

Article

Mann et al Chembiochem. 2024 May 2;25(9):e202400007

Insert

Cry2 - mCherry - Profilin (Cry2, PRF1 Mouse, Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

Ser 138 (sometimes numbered 137) mutated to Ala

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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