We narrowed to 1,042 results for: Psp

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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DGP104- pSP64 Poly(A)700

Plasmid

#70098

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

700bp insert from B. subtilis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP105- pSP64 Poly(A)900

Plasmid

#70100

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

900bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pSP64T XAR1 DNM1 (DM#102)

Plasmid

#15042

Depositor

Douglas Melton

Article

Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

Insert

Xenopus activin receptor 1 (xstk2 Frog)

Expression

Bacterial

Mutation

Dominant negative mutant

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64T Activin betaB (DM#108)

Plasmid

#15045

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

Insert

Activin Beta b (inhbb Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pspCas9-POLI-ST2-com vector

Plasmid

#176234

Purpose

Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Type

Empty backbone

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

Plasmid

#165082

Purpose

gRNA 1 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSP64T3 BMP2 pro Vg1 (DM#119)

Plasmid

#15071

Depositor

Douglas Melton

Article

Thomsen et al Cell. 1993 Aug 13. 74(3):433-41.

Insert

BMP2-Vg1

Use

Sp6 expression for use in xenopus

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

Plasmid

#68280

Purpose

SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Mkk2DS NLS mCherry (MKK2 Budding Yeast)

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSpliceExpress-KRAS-Exon2

Plasmid

#256555

Purpose

pSpliceExpress splicing reporter to measure inclusion or skipping of exon 2 of KRAS

Depositor

Matthew Meyerson

Article

Walter et al bioRxiv [Preprint]. 2025 Sep 18:2025.03.

Insert

KRAS Exon 2 (KRAS Human)

Expression

Mammalian

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pSpliceExpress-KRAS-Exon3

Plasmid

#256556

Purpose

pSpliceExpress splicing reporter to measure inclusion or skipping of exon 3 of KRAS

Depositor

Matthew Meyerson

Article

Walter et al bioRxiv [Preprint]. 2025 Sep 18:2025.03.

Insert

KRAS Exon 3 (KRAS Human)

Expression

Mammalian

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_R44K/E126Q/E179Q

Plasmid

#223819

Purpose

Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

R44K, E126Q, E179Q

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Grx1-roGFP2-PSPC1

Plasmid

#236549

Purpose

Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in paraspeckles.

Depositor

Pablo Rivera-Fuentes

Insert

Grx1-roGFP2-PSPC1 (PSPC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP-RB1sg

Plasmid

#229857

Purpose

single guide RNA targeting human RB1 gene

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

RB1sgRNA (RB1 Human)

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5-S88E

Plasmid

#174227

Purpose

Expresses human MDA5 S88E mutant

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S88E

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

Plasmid

#204080

Purpose

Type 0 promoter part

Depositor

Mikhail Shapiro

Insert

TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter

Expression

Bacterial

Mutation

None

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

Plasmid

#197421

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

Plasmid

#197422

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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