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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,611 results for: Sp1

Showing: 421 - 440 of 1611 results

  2. pHis-TEV-Nsp15-E266Q

    Plasmid
    #218803
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    E266Q (G to C)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218803-plasmid-map-sequence-id-433302

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  3. pHis-TEV-Nsp15-V66L

    Plasmid
    #219524
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    V66L (G to C)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219524-plasmid-map-sequence-id-433377

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  4. pHis-TEV-Nsp15-A81V

    Plasmid
    #219525
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    A81V (C to T)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219525-plasmid-map-sequence-id-433375

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  5. pHis-TEV-Nsp15-D183N

    Plasmid
    #219526
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    D183N (G to A)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219526-plasmid-map-sequence-id-433305

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  6. pHis-TEV-Nsp15-H234A

    Plasmid
    #219529
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    H234A (CAC to GCG)
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    219529-plasmid-map-sequence-id-433314

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  10. pT7-35aaNSP1SA11-Green Lantern(wt)

    Plasmid
    #220821
    Purpose
    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the Green Lantern wt protein carrying an ER-localization signal at the N- and C-termini
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1-Green Latern(wt)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220821-plasmid-map-sequence-id-443937

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  11. pT7-35aaNSP1SA11-Green Lantern(io)

    Plasmid
    #220822
    Purpose
    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the recoded Green Lantern protein carrying an ER-localization signal at the N- and C-termini
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1-Green Latern(io)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220822-plasmid-map-sequence-id-443932

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  12. pT7-NSP1delta103CSA11-P2A-GFP

    Plasmid
    #220814
    Purpose
    Express separately the Rotavirus SA11 delta103C NSP1 protein and the sfGFP (Pedelacq et al., 2006)
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 NSP1delta103C-P2A-GFP
    Use
    Other
    Tags
    Expression
    Mutation
    Lacks C-terminal 103 aa
    Promoter
    Available since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220814-plasmid-map-sequence-id-443928

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Showing: 421 - 440 of 1611 results