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pET_MBP-RGG-RGG

Plasmid

#124946

Purpose

Expression of MBP-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

MBP-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His and MBP

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGC31

Plasmid

#19678

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)-L4440

Use

Gateway Cloning and RNAi

Available Since

Feb. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pJA258

Plasmid

#21511

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::citrine(w introns)::tbb-2 3'UTR

Use

Gateway Cloning

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pSEM255 - sgRNA mosTI Pmlc-2_GFP

Plasmid

#159835

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI Pmlc-2_GFP

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_SZ1-RGG-GFP-RGG

Plasmid

#124932

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSR02

Plasmid

#69149

Purpose

Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD4 GFP

Plasmid

#19181

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

Green fluorescent protein

Tags

FLAG and daf-12 N-term 328 amino acids

Expression

Worm

Mutation

S65C, contains synthetic introns for C. elegans

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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punc-119cR

Plasmid

#21596

Depositor

Al Fisher

Article

Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

Insert

unc-119:mCherry (unc-119 Nematode)

Use

Adds unc-119 marker to amp resistant plasmids

Tags

mCherry

Expression

Worm

Mutation

N/A

Available Since

Aug. 26, 2009

Availability

Academic Institutions and Nonprofits only

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ploxP unc-119

Plasmid

#19185

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

unc-119 (unc-119 Nematode)

Use

Recombineering

Mutation

contains loxP site just 5' to ApaI site

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEG408

Plasmid

#40086

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (aa 345 - 468) (mex-5 Nematode)

Tags

Dendra2 PFAF, S-peptide, and TEV protease cleavag…

Expression

Worm

Mutation

contains aa 345 - 468

Promoter

pie-1

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Article

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Tags

GFP

Expression

Worm

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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BiP 2: C. elegans BiP (hsp-4)-GFP

Plasmid

#21896

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

BiP (hsp-4 Nematode)

Tags

GFP

Expression

Worm

Mutation

Fusion between the GFP and hsp-4 takes place at A…

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pOD2087-trnDEG

Plasmid

#89369

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Pmec-18

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBN260

Plasmid

#86722

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-3::FLP D5

Expression

Worm

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN278

Plasmid

#86724

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmec-7::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pPD162.21

Plasmid

#27511

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pmyo-3::dsRed::let-858_3'UTR

Expression

Worm

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCFJ676 - MCS(ttTi4348, I, unc-18(+))

Plasmid

#34872

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available Since

Feb. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCM1.109

Plasmid

#19597

Depositor

Geraldine Seydoux

Insert

pgl-3 prom in PDONRP4P1R

Expression

Worm

Available Since

Jan. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pJF5

Plasmid

#139031

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

gfp (no STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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