Addgene

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OccludinMut_Flag

Plasmid

#86043

Purpose

Full length Occludin with mutation of PPPY domain at AA9-12 to PAAY

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

OccludinMut (OCLN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

PPPY domain at AA9-12 to PAAY; I52T and Q365P

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCHFLAGGR

Plasmid

#1279

Depositor

Susan Lindquist

Article

Kimura et al Science 1995 Jun 2;268(5215):1362-5.

Insert

GR (Nr3c1 Rat)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRC2_3xflag_NRF2(RR)

Plasmid

#136522

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Entry vector for gateway cloning

Tags

3x FLAG

Expression

Mutation

This plasmid is developed by mutating 3 base pair…

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWzl_neo_DEST_flag_PIK3CA

Plasmid

#45308

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_ARPM1

Plasmid

#45259

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

ARPM1 (ACTRT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_BFP

Plasmid

#45302

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1mLCAD_8KR_FLAG

Plasmid

#24485

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

Plasmid

#231372

Purpose

Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Use

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_FLAG_hSF3B1

Plasmid

#176590

Purpose

Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

FLAG3

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG

Plasmid

#223895

Purpose

4G-cloning donor for C-terminal FLAG tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

FLAG

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTFG011_Spike_Parental_D614G_3xFlag

Plasmid

#191571

Purpose

SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G mutation. Last 19 amino acids truncated.

Promoter

CMV

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN001M-T

Plasmid

#218078

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001M-T

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN001T-N

Plasmid

#218077

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001T-N

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN006A-P

Plasmid

#218079

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN001K-M

Plasmid

#218084

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001K-M

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN004M-4

Plasmid

#218082

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN004M-4

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN008H-P

Plasmid

#218081

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN008H-P

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPFLAGH_BN0059-C

Plasmid

#218083

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN0059-C

Use

Tags

FLAG-His and PelB

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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