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pRSET FLII12Pglu-700uDelta6

Plasmid

#13568

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 6aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmOrange

Plasmid

#37139

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human alpha-actinin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pmRuby2-CrowdingLinker-Clover

Plasmid

#171058

Purpose

Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

Crowdedness-sensitive linker domain

Tags

mClover2 and mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available Since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2paCRY2

Plasmid

#129651

Purpose

2P-activatable CRY2

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-beta-Actin

Plasmid

#37136

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human beta-actin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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At1R-P2A-mCherry

Plasmid

#112934

Purpose

Plasmid co-producing the AT1R receptor and a mCherry

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

AT1R

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pVimentin-LSSmOrange

Plasmid

#37134

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytochrome C oxidase subunit VIII and human…

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GdT

Plasmid

#87361

Purpose

Expresses GdT, a dimeric green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GdT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2paCRY2(D387A)

Plasmid

#129652

Purpose

2P-activatable CRY2 with the FAD-disrupting mutation

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2(D387A)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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ROZA

Plasmid

#64198

Purpose

"Reporter of Zap-70 Activity" Fluorescent reporter of ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPZP FLIPmal-25u

Plasmid

#14476

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPmal

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

W230A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pRSET FLII12Pglu-600uDelta4

Plasmid

#13567

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 4aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pPZP FLIPmal-control

Plasmid

#14477

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPmal

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

W340A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pSIn-H3K9ac Y97F BS

Plasmid

#235132

Purpose

Mutant (negative control) Y97F biosensor for pSin-H3K9ac Full

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1Y97F-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Mutation

BD1Y97F

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

Plasmid

#209089

Purpose

mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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