We narrowed to 2,524 results for: organization Addgene

1. pUBC_V5_MS2-mVenus

   Plasmid

   #107606

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   MS2-mVenus
   

   Use

   Synthetic Biology

   Tags

   V5 and mVenus

   Expression

   Mutation

   MS2: V75E/A81G

   Promoter

   human UBC

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF-pIgH-ESCargo(FTV,Orange)

   Plasmid

   #140161

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-miniSOG-N1

   Plasmid

   #54803

   Purpose

   Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Type

   Empty backbone

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pME-APEX2-NS

   Plasmid

   #67669

   Purpose

   Gateway entry clone containing APEX2 with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2
   

   Use

   Gateway multisite middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUBC_HA_PP7-mCherry

   Plasmid

   #107605

   Purpose

   Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   PP7-mCherry
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   hUBC

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. miniSOG-Rab5a-7

   Plasmid

   #57774

   Purpose

   Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Rab5a
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pKmyc-Borg3

   Plasmid

   #141074

   Purpose

   Expresses Borg3 (Cdc42ep5) in mammalian cells

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

   Insert

   Borg3 (Cdc42ep5 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. K401-iLid

   Plasmid

   #122484

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   Kinesin 1-401 iLid His tag
   

   Use

   Tags

   His6 and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Promoter

   T7

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-AKT-PH

   Plasmid

   #67674

   Purpose

   Gateway entry clone containing AKT-PH with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   AKT-PH
   

   Use

   Gateway multisite middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMTTH+eGFP-bromo5(N140A)

    Plasmid

    #157806

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5(N140A)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    N140A

    Promoter

    pTAC

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKI-cjFOXL2-coemiRFP670-PGK-Puro

    Plasmid

    #186168

    Purpose

    Knock-in gene targeting in marmoset cells at FOXL2 locus

    Depositor

    Sho Yoshimatsu

    Article

    Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

    Insert

    emiRFP670
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6_T7_INT[6xminimal_Puf1]

    Plasmid

    #107608

    Purpose

    Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    6x minimal Puf1 motif
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. H6-epsin 1 (144-575)

    Plasmid

    #63008

    Purpose

    expresses the adaptor protein epsin that lacks its ENTH-domain in bacteria

    Depositor

    Ernst Ungewickell

    Article

    Kalthoff et al J Biol Chem. 2002 Mar 8;277(10):8209-16.

    Insert

    Epsin 1 without ENTH domain (Epn1 Rat)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

14. mCherry-miniSOG-Peroxisomes-2

    Plasmid

    #54804

    Purpose

    Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    Peroximal Targeting Signal 1 (PTS1) and mCherry-m…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. 5mCUC1 pGreenII0129

    Plasmid

    #12067

    Depositor

    David Bartel

    Article

    Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

    Insert

    CUC1 mutant (CUC1 Mustard Weed)
    

    Use

    Plant transformation via agrobacterium

    Tags

    Expression

    Mutation

    miR-resistant CUC1. Made by introducing five sile…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA6/ERGIC2-WT

    Plasmid

    #51479

    Purpose

    Expresses ERGIC2-WT in mammalian cells

    Depositor

    Simon Kwok

    Article

    Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.

    Insert

    ERGIC2-WT (ERGIC2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCS2 DN-xIGFR

    Plasmid

    #24699

    Depositor

    Edward De Robertis

    Article

    Pera et al Mech Dev. 2002 Aug . 116(1-2):169-72.

    Insert

    Dominant negative xIGFR (igf1r Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal truncated version of the Xenopus IGF t…

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

18. PA-mCherry-miniSOG-Lifeact-7

    Plasmid

    #54846

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry-miniSOG-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pME-BTK-PH

    Plasmid

    #67673

    Purpose

    Gateway entry clone containing BTK-PH with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    BTK-PH-NS
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-miniSOG-Rab5a-7

    Plasmid

    #55094

    Purpose

    Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Rab5a
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only