We narrowed to 5,806 results for: pCas

1. pCAG_mRuby2_smFP FLAG

   Plasmid

   #59760

   Purpose

   encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   mRuby2_smFP_FLAG
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

   Plasmid

   #198863

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human TERT gene

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   TERT sgRNA (TERT Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-scFvGCN4sfGFPTET1CD

   Plasmid

   #82561

   Purpose

   Expression vector for antibody-sfGFP-TET1CD.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   scFvGCN4sfGFPTET1CD
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-N2cG

   Plasmid

   #100811

   Purpose

   express rabies virus CVS N2c strain glycoprotein gene (N2cG)

   Depositor

   Ian Wickersham

   Insert

   rabies virus CVS N2c strain glycoprotein gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCaggs-mGold2s

   Plasmid

   #231763

   Purpose

   Expression of mGold2s in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2s
   

   Expression

   Mammalian

   Mutation

   mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Actin promoter with CMV enhancer

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_PCP-T5Exo

   Plasmid

   #214736

   Purpose

   encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_PCP-T5Exo_bGH
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCambia1300-PacI

   Plasmid

   #44183

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGs-BFP

    Plasmid

    #127348

    Purpose

    Expresses BFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Blue Fluorescent Protein
    

    Expression

    Mammalian

    Promoter

    CAGGS Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAD39_mIRG1_4-462_pvp008

    Plasmid

    #124873

    Purpose

    Expresses mouse CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (Acod1 Mouse)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 463-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCalN-ddGFP-A

    Plasmid

    #40290

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    Calnexin (CANX Human)
    

    Tags

    ddGFP-A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. KRAB-dSpCas9

    Plasmid

    #205248

    Purpose

    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAX APLP1

    Plasmid

    #30141

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Like Protein 1 (APLP1 Human)
    

    Expression

    Mammalian

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

15. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG/HA-hBIG2

    Plasmid

    #226254

    Purpose

    Expresses N-terminally HA-tagged hBIG2 in mammalian cells

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-eGFP-pA

    Plasmid

    #107281

    Purpose

    donor vector backbone with constitutive eGFP

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG2IG-Hmga1a

    Plasmid

    #122293

    Purpose

    Overexpression for mouse Hmga1a

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Hmga1a (Hmga1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-ASAP3ER

    Plasmid

    #195356

    Purpose

    ASAP3ER under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-hCas9

    Plasmid

    #51142

    Purpose

    Expresses hCas9 under the CAG promoter for CRISPR

    Depositor

    Izuho Hatada

    Article

    Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

    Insert

    hCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only