We narrowed to 4,717 results for: ARA-2

1. pJB02

   Plasmid

   #111959

   Purpose

   Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Inserts

   mEGFP
   eDHFR
   

   Tags

   DHFR

   Expression

   Bacterial

   Mutation

   eDHFR;residues 2-159 and enhanced GFP with monome…

   Promoter

   T7

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ppBAalbNixE1E2 OpIE2-GFP

   Plasmid

   #173505

   Purpose

   transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Inserts

   Nix masculinisation gene
   EGFP
   

   Expression

   Insect

   Mutation

   Nix isoforms with exons 1, 2 separated by intron 1

   Promoter

   OpIE2 and own

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-24p3/LCN2-282-LUC

   Plasmid

   #46865

   Purpose

   murine 24p3 minimal promoter as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Promoter

   24p3 minimal promoter

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET28a Gx-NL2

   Plasmid

   #169107

   Purpose

   Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc
   

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-24p3/LCN2-145-LUC

   Plasmid

   #46866

   Purpose

   murine 24p3 minimal promoter as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Promoter

   24p3 promoter (inactive)

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

6. DoS3 plasmid

   Plasmid

   #244471

   Purpose

   Containing RP4 oriT and Cas9 module, for eliminating RP4 plasmid.

   Depositor

   Lingchong You

   Article

   Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

   Insert

   I-SceI enzyme (CSS3 Synthetic)
   

   Use

   Synthetic Biology

   Promoter

   araBAD promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB_ROSA26_SpCas9_gRNAs

   Plasmid

   #174703

   Purpose

   Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

   Depositor

   Beverly L. Davidson

   Article

   Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

   Insert

   SpCas9 dual gRNAs
   

   Use

   AAV

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLKO.1-Cd155 shRNA1

   Plasmid

   #166487

   Purpose

   Mouse Cd155 RNAi

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   shRNA for mouse Cd155 (Pvr Mouse)
   

   Use

   Lentiviral

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-N1-MOB1

   Plasmid

   #166477

   Purpose

   Expresses fusion of mCherry and MOB1

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mCherry, MOB1 (MOB1A Human)
   

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. retro-gfpIkkb-puro vector

    Plasmid

    #58251

    Purpose

    Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Ikkβ (Ikbkb Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. ppBAalbNixE1E2 PUb-GFP

    Plasmid

    #173667

    Purpose

    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Inserts

    Nix masculinisation gene
    EGFP
    

    Expression

    Insect

    Mutation

    Nix isoforms with only exons 1, 2 separated by in…

    Promoter

    Aedes aegypti Polyubiquitin and cloned Nix promot…

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pBEL2251

    Plasmid

    #170403

    Purpose

    For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-717, replace with 225-249)

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

    Plasmid

    #78507

    Purpose

    Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Gads SH3 SH2 SH3 domain (GRAP2 Human)
    

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-Blast POLE1 N-ISCmut

    Plasmid

    #160803

    Purpose

    Express N-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 N-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXS-IRES-Blast POLE1 C-ISCmut

    Plasmid

    #160804

    Purpose

    Express C-terminal ISC mutant POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLE1 C-ISCmut (POLE Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    shPOLE_1 and shPOLE2 resistant, C2221S, C2224S, C…

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXS-IRES-Blast POLD1 ISCmut

    Plasmid

    #160806

    Purpose

    Express ISC mutant POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLD1 ISCmut (POLD1 Human)
    

    Use

    Retroviral

    Mutation

    Codon optimized C-terminal 230 amino acids, C1058…

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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