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pAW211

Plasmid

#113233

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGA

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->AACGA

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW210

Plasmid

#113189

Purpose

E. coli expression vector for untagged E. coli Cas1 H208A + Cas2 + CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

H208A

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAW216

Plasmid

#113238

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutant

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->ggtTg

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

E10A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Nqo1

Plasmid

#105992

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Nqo1 (Nqo1 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pACT2-Dhr1

Plasmid

#100003

Purpose

Express yeast Dhr1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Dhr1 (ECM16 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Dhr1

Plasmid

#100002

Purpose

Express yeast Dhr1 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Dhr1 (ECM16 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pAS2-1-Mrt4

Plasmid

#99997

Purpose

Express yeast Mrt4 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Mrt4 (MRT4 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Mrt4

Plasmid

#99998

Purpose

Express yeast Mrt4 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Mrt4 (MRT4 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Esf1

Plasmid

#99988

Purpose

Express yeast Esf1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Utp15

Plasmid

#99978

Purpose

Express yeast Utp15 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Utp15

Plasmid

#99977

Purpose

Express yeast Utp15 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221-Cka2

Plasmid

#99980

Purpose

Shuttle yeast Cka2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka2 (CKA2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp16

Plasmid

#99985

Purpose

Shuttle yeast Utp16 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp16 (BUD21 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Zfhx4

Plasmid

#84324

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zfhx4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Zfhx4 (Zfhx4 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Phtf1

Plasmid

#84318

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Phtf1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Phtf1 (Phtf1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Tgif1

Plasmid

#74359

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tgif1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

TGFB-induced factor homeobox 1 (Tgif1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zhx1

Plasmid

#74364

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

zinc fingers and homeoboxes 1 (Zhx1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Six1

Plasmid

#74358

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

sine oculis-related homeobox 1 (Six1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pbx3

Plasmid

#74352

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

pre B cell leukemia homeobox 3 (Pbx3 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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